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Inhibitory activity and antioomycete mechanism of citral against Phytophthora capsici.
Cui, Kaidi; Wang, Yinan; Wang, Mengke; Zhao, Te; Zhang, Fulong; He, Leiming; Zhou, Lin.
Afiliación
  • Cui K; College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan, China; Henan Key Laboratory of Creation and Application of New Pesticide, Henan Agricultural University, No. 63, Agricultural Road, Zhengzhou 450002, Henan, China; Henan Research Center of Green Pesticide Engineerin
  • Wang Y; Center for Biological Invasions, Shenyang University, Shenyang 110044, Liaoning, China.
  • Wang M; College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan, China; Henan Key Laboratory of Creation and Application of New Pesticide, Henan Agricultural University, No. 63, Agricultural Road, Zhengzhou 450002, Henan, China; Henan Research Center of Green Pesticide Engineerin
  • Zhao T; College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan, China; Henan Key Laboratory of Creation and Application of New Pesticide, Henan Agricultural University, No. 63, Agricultural Road, Zhengzhou 450002, Henan, China; Henan Research Center of Green Pesticide Engineerin
  • Zhang F; Inner Mongolia Kingbo Biotech Co., Ltd., Bayan Nur 015200, Inner Mongolia, China.
  • He L; College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan, China; Henan Key Laboratory of Creation and Application of New Pesticide, Henan Agricultural University, No. 63, Agricultural Road, Zhengzhou 450002, Henan, China; Henan Research Center of Green Pesticide Engineerin
  • Zhou L; College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan, China; Henan Key Laboratory of Creation and Application of New Pesticide, Henan Agricultural University, No. 63, Agricultural Road, Zhengzhou 450002, Henan, China; Henan Research Center of Green Pesticide Engineerin
Pestic Biochem Physiol ; 204: 106067, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39277383
ABSTRACT
The natural terpenoid citral has antifungal activity against multiple fungi, but its bioactivity against oomycetes is unclear. Therefore, this study investigated the antioomycete activity and mechanism of citral against Phytophthora capsici, a highly destructive invasive oomycete. Results showed that citral not only had a great inhibition on the mycelial growth of P. capsici (EC50 = 94.15 mg/L), but also had a significant inhibition on multiple spores, such as sporangia formation, zoospore discharge and zoospore germination. Citral at 4000 mg/L exhibited favorable protective (73.33%) and curative efficacy (55.11%) against pepper Phytophthora blight. Citral significantly damaged the hyphal morphology, disrupted the cell membrane integrity, increased the permeability of cell membrane, and increased the glycerol content in P. capsici. A total of 250 upregulated and 288 downregulated proteins were identified in iTRAQ-based quantitative proteomic analysis. Downregulated proteins were mostly enriched in pathways of ABC transporters, cyanoamino acid metabolism and starch and sucrose metabolism, suggesting an inhibition of citral on transmembrane transporter (e.g., ABC transporters) and pathogenicity (e.g., ß-glucosidases) proteins. Upregulated proteins were enriched in biosynthesis of unsaturated fatty acids, pyruvate metabolism and glycolysis/gluconeogenesis, suggesting an activation of citral on energy generation proteins, including acyl-CoA oxidase, D-lactate dehydrogenase, pyruvate kinase, acetyl-CoA synthetase and phosphoenolpyruvate carboxykinase. Biochemical and iTRAQ analysis suggested that cell membrane may be the target of citral in P. capsici.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Phytophthora / Monoterpenos Acíclicos Idioma: En Revista: Pestic Biochem Physiol Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Phytophthora / Monoterpenos Acíclicos Idioma: En Revista: Pestic Biochem Physiol Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos