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Role of Enterococcus mundtii in gut of the tomato leaf miner (Tuta absoluta) to detoxification of Chlorantraniliprole.
Chen, Yao; Chen, Yaping; Zhang, Yibo; Sun, Zhongxiang; Li, Yahong; Ding, Jiasheng; Zhang, Guifen; Du, Ewei; Zi, Xiaoyan; Tian, Chaoxin; Zhao, Wenyuan; Gui, Furong.
Afiliación
  • Chen Y; State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
  • Chen Y; State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China. Electronic address: cyp83@ynau.edu.cn.
  • Zhang Y; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Invasive Alien Species Control of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: zhangyibo@caas.cn.
  • Sun Z; State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China. Electronic address: szx@ynau.edu.cn.
  • Li Y; Yunnan Plant Protection and Quarantine Station, Kunming 650034, China.
  • Ding J; Plant Protection and Quarantine Station, Dehong Prefecture, Mangshi 678400, Yunnan Province, China.
  • Zhang G; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Invasive Alien Species Control of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: zhangguifen@caas.cn.
  • Du E; State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
  • Zi X; State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
  • Tian C; State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
  • Zhao W; State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
  • Gui F; State Key Laboratory of Conservation and Utilization of Biological Resources of Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China. Electronic address: guifr@ynau.edu.cn.
Pestic Biochem Physiol ; 204: 106060, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39277378
ABSTRACT
Chlorantraniliprole (CAP) is applied worldwide for the control of caterpillars (Lepidoptera). However, with the overuse of CAP, the resistance problem in pest control is becoming increasingly serious. Recent studies have indicated a central role of the gut symbiont in insect pest resistance to pesticides and these may apply to the tomato leaf miner Tuta absoluta, is one of the most destructive insects worldwide. Here, we successfully isolated seven strains of tolerant CAP bacterium from the CAP-resistant T. absoluta gut, of which Enterococcus mundtii E14 showed the highest CAP tolerance, with a minimum inhibitory concentration (MIC) of 1.6 g/L and CAP degradation rate of 42.4%. Through transcriptomics and metabolism analysis, we studied the detoxification process of CAP by the E. mundtii E14, and found that CAP can be degraded by E. mundtii E14 into non-toxic compounds, such as 3,4-dihydroxy-2-(5-hydroxy-3,7-dimethylocta-2,6-dien-1-yl) benzoic acid and 2-pyridylacetic acid. Additionally, 2-pyridylacetic acid was detected both intracellular and extracellular in E. mundtii E14 treated with CAP. Meanwhile, we identified 52 up-regulated genes, including those associated with CAP degradation, such as RS11670 and RS19130. Transcriptome results annotated using KEGG indicated significant enrichment in up-regulated genes related to the glyoxylate cycle, nitrogen metabolism, and biosynthesis of secondary metabolites. Additionally, we observed that reinfection with E. mundtii E14 may effectively enhance resistance of T. absoluta to CAP. The LC50 values of the antibiotic treatment population of T. absoluta reinfection with E. mundtii E14 is 0.6122 mg/L, which was 18.27 folds higher than before reinfection. These findings offer new insights into T. absoluta resistance to CAP and contribute to a better understanding of the relationship between insecticide resistance and gut symbionts of T. absoluta, which may play a pivotal role in pest management.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enterococcus / Ortoaminobenzoatos / Insecticidas Límite: Animals Idioma: En Revista: Pestic Biochem Physiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enterococcus / Ortoaminobenzoatos / Insecticidas Límite: Animals Idioma: En Revista: Pestic Biochem Physiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos