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Sulfated polysaccharide from Apostichopus japonicus viscera exhibits anti-inflammatory properties in vitro and in vivo.
Ye, Jing; Zheng, Linjing; Pan, Weipeng; Huang, Yayan; Zhang, Na; Yang, Dongda; Yang, Yucheng; Zheng, Bingde; Zhang, Xueqin; Xiao, Meitian.
Afiliación
  • Ye J; College of Chemical Enginering, Huaqiao University, Xiamen 362021, China; Engineering Technology Research Center for Comprehensive Utilization of Marine Biological Resources in Xiamen, Huaqiao University, Xiamen 361021, China. Electronic address: yejenny@hqu.edu.cn.
  • Zheng L; College of Mechanical Engineering and Automation, Huaqiao University, Xiamen, Fujian 361021, China.
  • Pan W; College of Chemical Enginering, Huaqiao University, Xiamen 362021, China.
  • Huang Y; College of Chemical Enginering, Huaqiao University, Xiamen 362021, China; Engineering Technology Research Center for Comprehensive Utilization of Marine Biological Resources in Xiamen, Huaqiao University, Xiamen 361021, China.
  • Zhang N; College of Chemical Enginering, Huaqiao University, Xiamen 362021, China; Engineering Technology Research Center for Comprehensive Utilization of Marine Biological Resources in Xiamen, Huaqiao University, Xiamen 361021, China.
  • Yang D; College of Chemical Enginering, Huaqiao University, Xiamen 362021, China.
  • Yang Y; College of Chemical Enginering, Huaqiao University, Xiamen 362021, China; Engineering Technology Research Center for Comprehensive Utilization of Marine Biological Resources in Xiamen, Huaqiao University, Xiamen 361021, China.
  • Zheng B; College of Chemical Enginering, Huaqiao University, Xiamen 362021, China; Engineering Technology Research Center for Comprehensive Utilization of Marine Biological Resources in Xiamen, Huaqiao University, Xiamen 361021, China.
  • Zhang X; College of Chemical Enginering, Huaqiao University, Xiamen 362021, China; Engineering Technology Research Center for Comprehensive Utilization of Marine Biological Resources in Xiamen, Huaqiao University, Xiamen 361021, China.
  • Xiao M; College of Chemical Enginering, Huaqiao University, Xiamen 362021, China; Engineering Technology Research Center for Comprehensive Utilization of Marine Biological Resources in Xiamen, Huaqiao University, Xiamen 361021, China.
Int J Biol Macromol ; : 135500, 2024 Sep 12.
Article en En | MEDLINE | ID: mdl-39276906
ABSTRACT
Polysaccharides from sea cucumbers are known for their biological activities, but little is known about those from sea cucumber viscera. The present study isolated a sulfated polysaccharide (SCVP-2) from the viscera of Apostichopus japonicas, which had a molecular weight of 209.1 kDa. SCVP-2 comprised 66.3 % total sugars, 2.1 % uronic acid, 4.5 % proteins, and 25.5 % sulfate groups, containing glucosamine, galactosamine, glucose, galactose, and fucose. FT-IR and NMR analyses identified SCVP-2 as a fucoidan sulfate with sulfation patterns of the fucose branches as Fuc2S, Fuc4S, and Fuc0S. SEM and AFM analyses showed irregular clusters and linear conformations. SCVP-2 demonstrated strong anti-inflammatory properties both in vitro and in vivo. In lipopolysaccharide (LPS)-induced inflammation in macrophage RAW264.7 cells, SCVP-2 significantly reduced nitric oxide (NO) and cytokine secretion (IL-1ß, IL-6, TNF-α). Additionally, it downregulated the expression of these cytokine genes. Furthermore, the anti-inflammatory mechanism of SCVP-2 was related to the inhibition of the MAPKs and NF-κB pathways. SCVP-2's anti-inflammatory capacity was confirmed in acute inflammation models, including xylene-induced ear swelling and acetic acid-induced peritoneal capillary permeability, and in high-fat diet-induced systemic low-grade chronic inflammation. In conclusion, SCVP-2 exhibits significant anti-inflammatory activity, suggesting its potential for development as a functional food ingredient or therapeutic agent for inflammation-related diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos