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Identification and functional analysis of lysophosphatidic acid phosphatase type 6 (ACP6) gene in golden pompano (Trachinotusovatus).
Qiu, Reng; Lei, Yang; Yang, Qiaoli; Zeng, Jian; Zhou, Yongcan; Sun, Bin; Sun, Yun.
Afiliación
  • Qiu R; Henan Provincial Engineering Laboratory of Insects Bio-reactor, Henan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Nanyang Normal University, Nanyang, 473061, China.
  • Lei Y; School of Breeding and Multiplication (Sanya Institute of Breedingand Multiplication), HainanUniversity, Sanya, Hainan, 572022, China.
  • Yang Q; Yantai Scibio Biotechnology Co., Ltd, Yantai, 264000, China.
  • Zeng J; School of Breeding and Multiplication (Sanya Institute of Breedingand Multiplication), HainanUniversity, Sanya, Hainan, 572022, China.
  • Zhou Y; School of Breeding and Multiplication (Sanya Institute of Breedingand Multiplication), HainanUniversity, Sanya, Hainan, 572022, China.
  • Sun B; Institute of Ocean Research, Fujian Polytechnic Normal University, Fuzhou, 350300, China.
  • Sun Y; School of Breeding and Multiplication (Sanya Institute of Breedingand Multiplication), HainanUniversity, Sanya, Hainan, 572022, China. Electronic address: syshui207@126.com.
Fish Shellfish Immunol ; 154: 109904, 2024 Sep 12.
Article en En | MEDLINE | ID: mdl-39276813
ABSTRACT
Golden pompano (Trachinotus ovatus), a marine farmed fish, is economically valuable in China. Lysophosphatidic acid phosphatase type 6 (ACP6) is a type of histidine acid phosphatase and plays an important role in regulating host inflammatory responses and anti-cancer effects in mammals. However, its function in teleost remains unknown. The present study aimed to investigate ACP6 function in golden pompano. ACP6 from golden pompano was identified, cloned, and named TroACP6. The open reading frame of TroACP6 was 1275 bp in length, encoding 424 amino acids. The TroACP6 protein shared high sequence identity (43.32%-90.57 %) with the ACP6 of other species. It contained a histidine phosphatase domain with the active site motif "RHGART" and the catalytic dipeptide HD (histidine and aspartate). Meanwhile, TroACP6 mRNA was widely distributed in the various tissues of healthy golden pompano, with the maximum expression in the head kidney. The function of TroACP6 was analyzed both in vitro and in vivo, and the results revealed that the purified recombinant TroACP6 protein exhibited optimum phosphatase activity at pH 6.0 and 50 °C in vitro. Meanwhile, upon Edwardsiella tarda challenge, TroACP6 expression in tissues increased significantly in vivo. In addition, TroACP6 overexpression enhanced the respiratory burst activity and superoxide dismutase activity of head kidney macrophages in vivo. Furthermore, the overexpression and knockdown of TroACP6 in vivo had a significant effect on bacterial infection. In summary, the study findings indicate that TroACP6 in golden pompano is involved in host defense against bacterial infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido