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The optimization and validation of a gas chromatography-mass spectrometry method to analyze the concentration of acetate, propionate and butyrate in human plasma or serum.
Vandermeulen, Greet; Rosseel, Riet; Chatonidi, Georgia; Deroover, Lise; Boets, Eef; Verbeke, Kristin.
Afiliación
  • Vandermeulen G; Translational Research in Gastrointestinal Disorders (TARGID), Department of chronic diseases and metabolism, Faculty of Medicine, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
  • Rosseel R; Translational Research in Gastrointestinal Disorders (TARGID), Department of chronic diseases and metabolism, Faculty of Medicine, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
  • Chatonidi G; Translational Research in Gastrointestinal Disorders (TARGID), Department of chronic diseases and metabolism, Faculty of Medicine, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
  • Deroover L; Translational Research in Gastrointestinal Disorders (TARGID), Department of chronic diseases and metabolism, Faculty of Medicine, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
  • Boets E; Translational Research in Gastrointestinal Disorders (TARGID), Department of chronic diseases and metabolism, Faculty of Medicine, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
  • Verbeke K; Translational Research in Gastrointestinal Disorders (TARGID), Department of chronic diseases and metabolism, Faculty of Medicine, KU Leuven, Herestraat 49, 3000 Leuven, Belgium. Electronic address: kristin.verbeke@kuleuven.be.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1247: 124299, 2024 Sep 07.
Article en En | MEDLINE | ID: mdl-39276608
ABSTRACT
Fermentation-derived short-chain fatty acids (SCFA)4 are potential mediators of the health benefits associated with dietary fiber intake. SCFA affect physiological processes locally in the gut and on distant organs via the systemic circulation. Since SCFA are used as energy source for colonocytes and substrate for the liver metabolism, their concentrations in the systemic circulation are low. Therefore, quantification of systemic SCFA requires sensitive analytical techniques. This article covers the optimization and validation of a gas chromatography-mass spectrometry method to measure systemic SCFA concentrations following derivatization with 2,4-difluoroaniline (DFA)5 and extraction in ethyl acetate. Sample preparation was optimized by varying the amount of DFA, coupling agent 1,3-dicyclohexylcarbodiimide, ethyl acetate and sodium bicarbonate, which is used to quench derivatization. In addition, evaporation of the samples using a vacuum concentrator resulted in less contamination, notably of acetate, compared to drying with N2 gas. The method showed excellent linearity with coefficient of variation (R2) > 0.99 and a good precision (relative standard deviation < 20 %) and accuracy. Finally, systemic concentrations of SCFA in human plasma samples could successfully be determined.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Países Bajos