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[Tujia medicine Toddalia asiatica improves synovial pannus in rats with collagen-induced arthritis through the PI3K/Akt signaling pathway].
Xiang, S; Zhang, Z; Jiang, L; Liu, D; Li, W; Bao, Z; Tian, R; Cheng, D; Yuan, L.
Afiliación
  • Xiang S; 1Hubei Enshi college, Enshi 445000, China.
  • Zhang Z; Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University, Enshi 445000, China.
  • Jiang L; Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University, Enshi 445000, China.
  • Liu D; Health Science Center, Hubei Minzu University, Enshi 445000, China.
  • Li W; Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University, Enshi 445000, China.
  • Bao Z; Health Science Center, Hubei Minzu University, Enshi 445000, China.
  • Tian R; Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University, Enshi 445000, China.
  • Cheng D; Health Science Center, Hubei Minzu University, Enshi 445000, China.
  • Yuan L; Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University, Enshi 445000, China.
Nan Fang Yi Ke Da Xue Xue Bao ; 44(8): 1582-1588, 2024 Aug 20.
Article en Zh | MEDLINE | ID: mdl-39276054
ABSTRACT

OBJECTIVE:

To investigate the therapeutic mechanism of Tujia medicine Toddalia asiatica alcohol extract (TAAE) for synovial pannus formation in rats with college-induced arthritis (CIA).

METHODS:

Sixty male SD rats were randomized into normal control group, CIA model group, TGT group, 3 TAAE treatment groups at low, medium and high doses (n=10). Except for those in the normal control group, all the rats were subjected to CIA modeling using a secondary immunization method and treatment with saline, TGT or TAAE by gavage once daily for 35 days. The severity of arthritis was assessed using arthritis index (AI) score, and knee joint synovium pathologies were examined with HE staining. Serum levels of TNF-α, IL-6, and IL-1ß were detected with ELISA; the protein expressions of PI3K, Akt, p-PI3K, p-Akt, VEGF, endostatin, HIF-1α, MMP1, MMP3, and MMP9 in knee joint synovial tissues were determined using Western blotting, and the mRNA expressions of TNF­α, IL-6, IL-1ß, VEGF, HIF-1α, PI3K, and Akt were detected with RT-PCR.

RESULTS:

Treatment of CIA rat models with TAAE and TGT significantly alleviated paw swelling, lowered AI scores, and reduced knee joint pathology, neoangiogenesis, and serum levels of inflammatory factors. TAAE treatment obviously increased endostatin protein expression, downregulated p-PI3K, p-Akt, MMP1, MMP3, MMP9, VEGF, and HIF-1α proteins, and reduced TNF­α, IL-6, IL-1ß, PI3K, Akt, VEGF, and HIF-1α mRNA levels in the synovial tissues, and these changes were comparable between high-dose TAAE group and TGT group.

CONCLUSION:

TAAE can improve joint symptoms and inhibit synovial pannus formation in CIA rats by regulating the expressions of HIF-1α, VEGF, endostatin, MMP1, MMP3, and MMP9 via the PI3K/Akt signalling pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Experimental / Transducción de Señal / Ratas Sprague-Dawley / Fosfatidilinositol 3-Quinasas / Factor A de Crecimiento Endotelial Vascular / Proteínas Proto-Oncogénicas c-akt / Subunidad alfa del Factor 1 Inducible por Hipoxia Límite: Animals Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Experimental / Transducción de Señal / Ratas Sprague-Dawley / Fosfatidilinositol 3-Quinasas / Factor A de Crecimiento Endotelial Vascular / Proteínas Proto-Oncogénicas c-akt / Subunidad alfa del Factor 1 Inducible por Hipoxia Límite: Animals Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China