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Changes in Plasma Concentration of Free Proteinogenic and Non-Proteinogenic Amino Acids in High-Performance Sprinters over a 6-Month Training Cycle.
Kusy, Krzysztof; Matysiak, Jan; Zarebska, Ewa Anna; Klupczynska-Gabryszak, Agnieszka; Ciekot-Soltysiak, Monika; Plewa, Szymon; Kokot, Zenon J; Derezinski, Pawel; Zielinski, Jacek.
Afiliación
  • Kusy K; Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Królowej Jadwigi Street 27/39, 61-871 Poznan, Poland.
  • Matysiak J; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, ul. Rokietnicka, 60-806 Poznan, Poland.
  • Zarebska EA; Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Królowej Jadwigi Street 27/39, 61-871 Poznan, Poland.
  • Klupczynska-Gabryszak A; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, ul. Rokietnicka, 60-806 Poznan, Poland.
  • Ciekot-Soltysiak M; Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, Królowej Jadwigi Street 27/39, 61-871 Poznan, Poland.
  • Plewa S; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, ul. Rokietnicka, 60-806 Poznan, Poland.
  • Kokot ZJ; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, ul. Rokietnicka, 60-806 Poznan, Poland.
  • Derezinski P; Faculty of Health Sciences, Calisia University, ul. Nowy Swiat, 4, 62-800 Kalisz, Poland.
  • Zielinski J; Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, ul. Rokietnicka, 60-806 Poznan, Poland.
J Clin Med ; 13(17)2024 Sep 06.
Article en En | MEDLINE | ID: mdl-39274513
ABSTRACT
Background/

Objectives:

Free amino acids substantially contribute to energy metabolism. Also, their profile may identify (over)training status and effectiveness. The long-term effects of speed-power training on plasma free amino acid (PFAA) profiles are not known. We aimed to observe variations in PFAA levels in high-performance sprinters in a six-month training cycle.

Methods:

Ten male athletes (24.6 ± 3.3 years) were examined during four training phases transition (1 month), general preparation (2 months), specific preparation (1 month), and pre-competition/competition (2 months). Venous blood was collected at rest, after exhaustive exercise, and recovery. Forty-two PFAAs were analyzed by the LC-ESI-MS/MS method.

Results:

Significant decreases in resting concentrations were observed between the transition and competition phases for glutamine (762 ± 117 vs. 623 ± 53 µmol∙L-1; p < 0.001, η2 = 0.47) and histidine (89 ± 15 vs. 75 ± 10 µmol∙L-1; p = 0.010, η2 = 0.27), whereas ß-alanine (30 ± 7 vs. 41 ± 9 µmol∙L-1; p = 0.024, η2 = 016) and sarcosine (3.6 ± 0.4 vs. 4.8 ± 0.6 µmol∙L-1; p = 0.006, η2 = 0.188) levels increased. Between the specific and competition phases, significant decreases in the resting levels of 1-methylhistidine (22.1 ± 19.4 vs. 9.6 ± 8.8 µmol∙L-1; p = 0.14, η2 = 0.19), 3-methylhistidine (7.1 ± 1.5 vs. 6.5 ± 1.6 µmol∙L-1; p = 0.009, η2 = 0.18), citrulline (40 ± 10 vs. 29 ± 4 µmol∙L-1; p = 0.05, η2 = 0.29), and ornithine (74 ± 15 vs. 56 ± 10 µmol∙L-1; p = 0.015, η2 = 185) were noticed. Also, for ß-alanine and sarcosine, the pattern of response to exercise strongly changed between the training phases. Blood ammonia levels at exhaustion decreased between the transition and competition phases (32 ± 4 vs. 23 ± 5 µmol∙L-1; p < 0.001, η2 = 0.67), while lactate, the phenylalanine-tyrosine ratio, the glutamine-glutamate ratio, hematological parameters, and cardiorespiratory indices remained at similar levels.

Conclusions:

Speed-power training seems to affect PFAAs involved in skeletal muscle metabolic pathways responsible for neutralizing toxic ammonia (glutamine, arginine, citrulline, ornithine), attenuating the deleterious effects of H+ ions (histidine, ß-alanine), and reducing exercise-induced protein breakdown (1- and 3-methylhistidine). Our findings suggest that sprint-oriented training supports metabolic pathways that are responsible for the removal of harmful metabolites produced during exercise.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Clin Med Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Clin Med Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza