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Comparison between USPIOs and SPIOs for Multimodal Imaging of Extracellular Vesicles Extracted from Adipose Tissue-Derived Adult Stem Cells.
Capuzzo, Arnaud M; Piccolantonio, Giusi; Negri, Alessandro; Bontempi, Pietro; Lacavalla, Maria A; Malatesta, Manuela; Scambi, Ilaria; Mariotti, Raffaella; Lüdtke-Buzug, Kerstin; Corsi, Mauro; Marzola, Pasquina.
Afiliación
  • Capuzzo AM; Department of Diagnostics and Public Health, University of Verona, Strada le Grazie, 8, 37134 Verona, Italy.
  • Piccolantonio G; Department of Engineering for Innovation Medicine, University of Verona, Strada le Grazie, 15, 37134 Verona, Italy.
  • Negri A; Department of Diagnostics and Public Health, University of Verona, Strada le Grazie, 8, 37134 Verona, Italy.
  • Bontempi P; Department of Engineering for Innovation Medicine, University of Verona, Strada le Grazie, 15, 37134 Verona, Italy.
  • Lacavalla MA; Department of Engineering for Innovation Medicine, University of Verona, Strada le Grazie, 15, 37134 Verona, Italy.
  • Malatesta M; Department of Chemical Science, University of Padova, Via Marzolo 1, 35131 Padova, Italy.
  • Scambi I; Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, Italy.
  • Mariotti R; Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, Italy.
  • Lüdtke-Buzug K; Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, Italy.
  • Corsi M; Institute of Medical Engineering, University of Luebeck, Ratzeburger Allee 160, 23562 Lübeck, Germany.
  • Marzola P; Fraunhofer Research Institution for Individualized and Cell-Based Medical Engineering IMTE, 23562 Lübeck, Germany.
Int J Mol Sci ; 25(17)2024 Sep 07.
Article en En | MEDLINE | ID: mdl-39273647
ABSTRACT
Adipose tissue-derived adult stem (ADAS) cells and extracellular vesicle (EV) therapy offer promising avenues for treating neurodegenerative diseases due to their accessibility and potential for autologous cell transplantation. However, the clinical application of ADAS cells or EVs is limited by the challenge of precisely identifying them in specific regions of interest. This study compares two superparamagnetic iron oxide nanoparticles, differing mainly in size, to determine their efficacy for allowing non-invasive ADAS tracking via MRI/MPI and indirect labeling of EVs. We compared a USPIO (about 5 nm) with an SPIO (Resovist®, about 70 nm). A physicochemical characterization of nanoparticles was conducted using DLS, TEM, MRI, and MPI. ADAS cells were labeled with the two nanoparticles, and their viability was assessed via MTT assay. MRI detected labeled cells, while TEM and Prussian Blue staining were employed to confirm cell uptake. The results revealed that Resovist® exhibited higher transversal relaxivity value than USPIO and, consequently, allows for detection with higher sensitivity by MRI. A 200 µgFe/mL concentration was identified as optimal for ADAS labeling. MPI detected only Resovist®. The findings suggest that Resovist® may offer enhanced detection of ADAS cells and EVs, making it suitable for multimodal imaging. Preliminary results obtained by extracting EVs from ADAS cells labeled with Resovist® indicate that EVs retain the nanoparticles, paving the way to an efficient and multimodal detection of EVs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagen por Resonancia Magnética / Tejido Adiposo / Células Madre Adultas / Nanopartículas de Magnetita / Vesículas Extracelulares / Nanopartículas Magnéticas de Óxido de Hierro Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagen por Resonancia Magnética / Tejido Adiposo / Células Madre Adultas / Nanopartículas de Magnetita / Vesículas Extracelulares / Nanopartículas Magnéticas de Óxido de Hierro Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza