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Molecular Characterization of Odorant-Binding Protein Genes Associated with Host-Seeking Behavior in Oides leucomelaena.
Zhao, Ning; Li, Kai; Ma, Huifen; Hu, Lianrong; Yang, Yingxue; Liu, Ling.
Afiliación
  • Zhao N; College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China.
  • Li K; College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China.
  • Ma H; Yunnan Academy of Forestry and Grassland, Kunming 650224, China.
  • Hu L; Yunnan Academy of Forestry and Grassland, Kunming 650224, China.
  • Yang Y; College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China.
  • Liu L; Yunnan Academy of Forestry and Grassland, Kunming 650224, China.
Int J Mol Sci ; 25(17)2024 Aug 30.
Article en En | MEDLINE | ID: mdl-39273382
ABSTRACT
The identification of odorant-binding proteins (OBPs) involved in host location by Oides leucomelaena (O. leucomelaena Weise, 1922, Coleoptera, Galerucinae) is significant for its biological control. Tools in the NCBI database were used to compare and analyze the transcriptome sequences of O. leucomelaena with OBP and other chemosensory-related proteins of other Coleoptera insects. Subsequently, MEGA7 was utilized for OBP sequence alignment and the construction of a phylogenetic tree, combined with expression profiling to screen for candidate antennae-specific OBPs. In addition, fumigation experiments with star anise volatiles were conducted to assess the antennae specificity of the candidate OBPs. Finally, molecular docking was employed to speculate on the binding potential of antennae-specific OBPs with star anise volatiles. The study identified 42 candidate OBPs, 8 chemosensory proteins and 27 receptors. OleuOBP3, OleuOBP5, and OleuOBP6 were identified as classic OBP family members specific to the antennae, which was confirmed by volatile fumigation experiments. Molecular docking ultimately clarified that OleuOBP3, OleuOBP5, and OleuOBP6 all exhibit a high affinity for ß-caryophyllene among the star anise volatiles. We successfully obtained three antennae-specific OBPs from O. leucomelaena and determined their high-affinity volatiles, providing a theoretical basis for the development of attractants in subsequent stages.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Escarabajos / Receptores Odorantes / Proteínas de Insectos / Simulación del Acoplamiento Molecular Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Escarabajos / Receptores Odorantes / Proteínas de Insectos / Simulación del Acoplamiento Molecular Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza