Your browser doesn't support javascript.
loading
Isolation and characterization of a multidrug-resistant Staphylococcus aureus infecting phage and its therapeutic use in mice.
Xiao, Zhen; Xu, Hongyi; Wang, Juan; Hu, Xueyuan; Huang, Xiumei; Song, Shiping; Zhang, Qingqing; Liu, Yanxin; Liu, Yaopeng; Liu, Na; Liu, Junhui; Zhao, Ge; Zhang, Xiyue; Li, Yuehua; Zhao, Jianmei; Wang, Junwei; Liu, Huanqi; Wang, Lin; Qu, Zhina.
Afiliación
  • Xiao Z; China animal health and epidemiology center, Qingdao 266032, China.
  • Xu H; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
  • Wang J; China animal health and epidemiology center, Qingdao 266032, China.
  • Hu X; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
  • Huang X; China animal health and epidemiology center, Qingdao 266032, China.
  • Song S; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
  • Zhang Q; China animal health and epidemiology center, Qingdao 266032, China.
  • Liu Y; China animal health and epidemiology center, Qingdao 266032, China.
  • Liu Y; China animal health and epidemiology center, Qingdao 266032, China.
  • Liu N; China animal health and epidemiology center, Qingdao 266032, China.
  • Liu J; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
  • Zhao G; China animal health and epidemiology center, Qingdao 266032, China.
  • Zhang X; College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
  • Li Y; China animal health and epidemiology center, Qingdao 266032, China.
  • Zhao J; China animal health and epidemiology center, Qingdao 266032, China.
  • Wang J; China animal health and epidemiology center, Qingdao 266032, China.
  • Liu H; China animal health and epidemiology center, Qingdao 266032, China.
  • Wang L; China animal health and epidemiology center, Qingdao 266032, China.
  • Qu Z; China animal health and epidemiology center, Qingdao 266032, China.
FEMS Microbiol Lett ; 3712024 Jan 09.
Article en En | MEDLINE | ID: mdl-39271451
ABSTRACT
In recent years, the emergence of multidrug-resistant bacteria has limited the selection of drugs for treating bacterial infections, reduced clinical efficacy, and increased treatment costs and mortality. It is urgent to find alternative antibiotics. In order to explore a new method for controlling methicillin-resistant Staphylococcus aureus (S. aureus), this study isolated and purified a multidrug-resistant S. aureus broad-spectrum phage JPL-50 from wastewater. JPL-50 belongs to the Siphoviridae family after morphological observation, biological characterization, and transmission electron microscopy (TEM) fragmentation spectrum analysis. It can cleave 84% of tested S. aureus (168/200), in which 100% of tested mastitis-associated strains (48/48) and 72.04% of MRSA strains (67/93) were lysed. In addition, it has an optimal growth temperature of about 30°C, a high activity within a wide pH range (pH 3-10), and an optimal multiplicity of infection of 0.01. The one-step growth curve shows a latent time of 20 min, an explosive time of 80 min. JPL-50 was 16 927 bp in length and was encoded by double-stranded DNA, with no genes associated with bacterial resistance or virulence factors detected. In a therapeutic study, injection of the phage JPL-50 once and for 7 times in 7 days protected 40% and 60% of the mice from fatal S. aureus infection, respectively. More importantly, JPL-50-doxycycline combination could effectively inhibit host S. aureus in vitro and reduce the use of doxycycline within 8 h. In conclusion, the bacteriophage JPL-50 has a wide lysis spectrum, high lysis rate, high tolerance to extreme environments, and moderate in vivo activity, providing ideas for developing multidrug-resistant S. aureus infections.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Fagos de Staphylococcus / Farmacorresistencia Bacteriana Múltiple / Staphylococcus aureus Resistente a Meticilina / Terapia de Fagos Límite: Animals Idioma: En Revista: FEMS Microbiol Lett Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Fagos de Staphylococcus / Farmacorresistencia Bacteriana Múltiple / Staphylococcus aureus Resistente a Meticilina / Terapia de Fagos Límite: Animals Idioma: En Revista: FEMS Microbiol Lett Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido