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Protocol for detecting lysosome quantity and membrane permeability in acute myeloid leukemia cell lines.
Huang, Kexiu; Jiang, Xinya; Du, Juan; Zeng, Hui.
Afiliación
  • Huang K; Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China. Electronic address: huang@stu2022.jnu.edu.cn.
  • Jiang X; Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China; Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
  • Du J; Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China. Electronic address: du.juan@u.nus.edu.
  • Zeng H; Department of Hematology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510180, China. Electronic address: androps2011@hotmail.com.
STAR Protoc ; 5(3): 103309, 2024 Sep 20.
Article en En | MEDLINE | ID: mdl-39269898
ABSTRACT
Lysosomal function and activity are essential to support cellular adaptation to multiple stresses. For example, certain drugs can induce increased lysosomal membrane permeability to exert their anti-cancer effects. Here, we present a protocol to evaluate the lysosome alterations induced by drug treatment. We first describe the steps for inducing lysosomal alterations in cultured cells. We then show how to quantify the number of lysosomes, assess the integrity of lysosomal membranes, and determine lysosomal membrane permeabilization by using galectin puncta assay. For complete details on the use and execution of this protocol, please refer to Jiang et al.1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / Permeabilidad de la Membrana Celular / Lisosomas Límite: Humans Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / Permeabilidad de la Membrana Celular / Lisosomas Límite: Humans Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos