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Advancing Lung Cancer Diagnosis through NH2-MON-SPME-GC-MS/MS: Enhanced Sensitivity in Aldehyde Biomarker Detection from Exhaled Breath.
Zhang, Feiran; Li, Pengfei; Lu, Yanke; Han, Yehong; Yan, Hongyuan.
Afiliación
  • Zhang F; Hebei Key Laboratory of Public Health Safety, College of Pharmaceutical Science, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China.
  • Li P; Hebei Key Laboratory of Public Health Safety, College of Pharmaceutical Science, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China.
  • Lu Y; Hebei Key Laboratory of Public Health Safety, College of Pharmaceutical Science, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China.
  • Han Y; Hebei Key Laboratory of Public Health Safety, College of Pharmaceutical Science, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China.
  • Yan H; Hebei Key Laboratory of Public Health Safety, College of Pharmaceutical Science, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China.
Anal Chem ; 2024 Sep 13.
Article en En | MEDLINE | ID: mdl-39269845
ABSTRACT
The sensitive detection of trace biomarkers in exhaled breath for lung cancer diagnosis represents a critical area of research in life analytical chemistry, with profound implications for early disease detection, therapeutic intervention, and prognosis monitoring. Despite its potential, the analytical process faces significant challenges due to the ultratrace levels of disease biomarkers present and the complex, high-humidity composition of exhaled breath. This study introduces a highly sensitive method for detecting aldehyde biomarkers in exhaled breath by integrating the use of amino-functionalized microporous organic networks (NH2-MON) as a solid-phase microextraction (SPME) fiber coating with gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) analysis. The method innovatively combines sample collection and extraction, achieving a dual-step enrichment process that significantly enhances both the enrichment efficiency and reproducibility of biomarker detection while effectively mitigating the interference caused by water vapor in exhaled breath. The NH2-MON, utilized as an SPME fiber coating, demonstrates exceptional enrichment capacity for five key aldehyde biomarkers, facilitating the development of a highly sensitive detection approach for these biomarkers in exhaled breath. Compared to previously reported methods, the proposed technique exhibits significantly lower limits of quantification, ranging from 0.77 to 11.89 pg mL-1, and achieves substantially higher enrichment factors, ranging from 9156- to 35723-fold. The practicality and feasibility of the method were validated through the analysis of exhaled breath samples from lung cancer patients, underscoring its potential application in the early diagnosis and monitoring of lung cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos