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LncRNA ILF3AS1, MMP3, and MMP9 as well as miRNA-212 as emerging novel biomarkers for childhood epilepsy.
Mohammed, Amena Rezk; Emam, Wafaa Abdelaziz; Mohammed, Shaymaa A; Abd Elalim, Alshaymaa A; Mansour, Eatemad Nabil Abdelhalim; Nasr, Haidy Mahmoud; Ghamry, Aya A; Alkhawagah, Sabah M; Fathy, Doaa Sadek Ahmed; Elattar, Rasha Sobhy; Abish, Yasser Gaber Ibrahim; Hussein, Abdullah; Zaghloul, Boshra Ahmed; Khairallah, Marwa K; Alharbi, Norah; Seif Eldin, Salwa; Dawood, Amal Fahmy; Sabet, Marwa A; Gamea, Marwa G; Elshishtawy Ibrahim, Suzan Eid; Mosa, Aliaa A; Dahpy, Marwa A.
Afiliación
  • Mohammed AR; Biochemistry Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Emam WA; Biochemistry Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Mohammed SA; Clinical Pathology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Abd Elalim AA; Clinical Pathology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Mansour ENA; Pediatrics Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Nasr HM; Pediatrics Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Ghamry AA; Medical Microbiology and Immunology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Alkhawagah SM; Medical Microbiology and Immunology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Fathy DSA; Community and Occupational Medicine Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Elattar RS; Neurology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Abish YGI; Radiodiagnosis Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
  • Hussein A; Radiodiagnosis and Intervention Radiology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Zaghloul BA; Radiodiagnosis and Intervention Radiology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Khairallah MK; Department of Internal Medicine, Faculty of Medicine, Assiut University, Asyut, Egypt.
  • Alharbi N; Internal Medicine Department, College of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
  • Seif Eldin S; Department of Basic Medical Sciences, College of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
  • Dawood AF; Department of Basic Medical Sciences, College of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
  • Sabet MA; Department of Microbiology and Immunology, Faculty of Pharmacy, Sphinx University, New Assiut, Egypt.
  • Gamea MG; Department of Pharmacology, Faculty of Medicine, Assiut University, Asyut, Egypt.
  • Elshishtawy Ibrahim SE; Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
  • Mosa AA; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Assiut University, Asyut, Egypt.
  • Dahpy MA; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Assiut University, Asyut, Egypt.
Front Mol Biosci ; 11: 1434023, 2024.
Article en En | MEDLINE | ID: mdl-39268188
ABSTRACT

Background:

Globally, approximately 70 million people suffer from epilepsy. Infants constitute a significant percentage of these cases. Hence, there is a significant need for better understanding of the pathophysiology of epilepsy through laboratory and radiological methods for early detection and optimized management. Interleukin enhancer binding factor 3 antisense RNA l (ILF3AS1) is a long non-coding RNA (lncRNA) that enhances the expressions of matrix metalloproteinase 3 (MMP3) and matrix metalloproteinase 9 (MMP9), which are considered to be epileptogenic.

Aim:

We aimed to assess the serum expressions of the lncRNAs ILF3AS1, MMP3, and MMP9 along with microRNA-212 (miRNA-212) as predictive biomarkers in children with epilepsy; we also assessed their correlations with magnetic resonance imaging (MRI) findings. Subjects and

Methods:

Fifty children with epilepsy and fifty healthy controls were considered in this study. Serum expressions of the lncRNA ILF3AS1 and miRNA-212 were estimated by quantitative real-time polymerase chain reaction (qPCR). Serum concentrations of MMP3 and MMP9 were estimated by enzyme-linked immunosorbent assay (ELISA) in parallel with MRI findings and different baseline biochemical parameters of all the subjects.

Results:

The results showed significantly higher levels of lncRNAs ILF3AS1, MMP3, and MMP9 as well as lower levels of miRNA-212 in children with epilepsy compared to the controls. The fold-change of miRNA-212 was a significant negative predictor (odds ratio = 0.153, p = 0.000). The receiver operating characteristic curves (Roc) showed that the areas under the curves for MMP3, MMP9, and lncRNA ILF3AS1 as well as the fold-change for miRNA-212 were 0.659, 0.738, 0.656, and 0.965, respectively. Brain lesions were detected in 15 patients (30%) with epilepsy, whereas the remaining 35 patients (70%) had normal results.

Conclusion:

Serum levels of the lncRNA ILF3AS1 among children with epilepsy were higher than those in the control group and were associated with upregulation of both MMP3 and MMP9 as well as downregulation of miRNA-212 expressions, suggesting their predictive utility in monitoring the development of epilepsy; this also means that a treatment plan focusing on the ILF3AS1/miRNA-212/MMP3/MMP9 axis could be an effective strategy for treating epilepsy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Mol Biosci Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Mol Biosci Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza