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Ammonia exposure impairs bone mineralization in zebrafish (Danio rerio) larvae.
Wang, Jun-Yi; Hsu, Hsuan-Yi; Liu, Sian-Tai; Lin, Chia-Hao.
Afiliación
  • Wang JY; Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan.
  • Hsu HY; Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan.
  • Liu ST; Department of Life Science, National Taiwan University, Taipei, Taiwan.
  • Lin CH; Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan; The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung, Taiwan. Electronic address: ch123@nkust.edu.tw.
Comp Biochem Physiol C Toxicol Pharmacol ; 287: 110040, 2024 Sep 10.
Article en En | MEDLINE | ID: mdl-39265966
ABSTRACT
Ammonia is a major pollutant of freshwater environments. Previous studies have indicated that ammonia exposure adversely affects the physiology of freshwater fish. However, its effect on bone mineralization in freshwater fish larvae remains unclear. In this study, zebrafish larvae were used as a model to investigate the effects of different ammonia levels (0, 2.5, 5, and 10 mM NH4Cl) on the survival rate, body length, and bone mineralization of fish. The survival rate of zebrafish embryos exposed to different NH4Cl concentrations for 8 days was not affected. In contrast, the body length and bone mineralization of zebrafish larvae at 8 days post fertilization (dpf) were significantly reduced at 5 and 10 mM NH4Cl exposure. Further investigations revealed that ammonia exposure decreased the mRNA expression of osteoblast-related genes and increased that of osteoclast-related genes. Additionally, exposure to 5 mM and 10 mM NH4Cl induced the production of reactive oxygen species (ROS). 10 mM-but not 5 mM-NH4Cl exposure reduced the calcium and phosphorus content in 8 dpf zebrafish larvae. In conclusion, ammonia exposure induces bone resorption, while decreasing the calcium and phosphorus content of the whole body and bone formation, resulting in impaired bone mineralization in fish larvae.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Comp Biochem Physiol C Toxicol Pharmacol Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Comp Biochem Physiol C Toxicol Pharmacol Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos