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A target-triggered colorimetric sensor for ultrasensitive detection of miRNAs based on self-powered three-dimensional DNA walker.
Li, Yingxue; Zhang, Tingrui; Bai, Gang; Chen, Mengchun; Lei, Xiaodong; Ye, Li; Yu, Hua; Fan, Zhichao; Yu, Tao.
Afiliación
  • Li Y; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China; Department of Cardiac Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China.
  • Zhang T; Department of Oncology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.
  • Bai G; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
  • Chen M; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
  • Lei X; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
  • Ye L; School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
  • Yu H; Department of General Surgery, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China. Electronic address: yuhua1232021@163.com.
  • Fan Z; Department of Oncology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China. Electronic address: fan-zhi-chao@163.com.
  • Yu T; Department of Cardiac Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China. Electronic address: yutaoxw@med.uestc.edu.cn.
Int J Biol Macromol ; 279(Pt 4): 135370, 2024 Nov.
Article en En | MEDLINE | ID: mdl-39265909
ABSTRACT
MicroRNAs (miRNAs) play an important role in the process of heart failure (HF) and are emerging biomarkers that can be used for the auxiliary diagnosis of HF. However, it is very challenging to accurately analyze the expression levels of trace miRNAs in complex clinical samples. Here, we developed an enzyme-free colorimetric sensor for the ultrasensitive detection of miRNA-423-5p (HF-associated miRNA) based on three-dimensional DNA walkers constructed from functional nucleic acids and gold nanoparticles (AuNPs). DNAzyme with cleavage activity was specifically activated by miRNA-423-5p to sustainably cleave the substrate, thereby releasing the trigger sequence to initiate the subsequent mismatched catalytic hairpin assembly (MCHA) cycle. Then, as the MCHA cycle proceeded to continuously expose the G-quadruplex (GQ) sequence, the sequence bound with hemin to form a large amount of GQ/hemin DNAzyme on the surface of the AuNPs, which rapidly catalyzed the chromogenic oxidation of 3,3',5,5'-tetramethylbenzidine to yield an amplified colorimetric signal readout. The colorimetric sensor exhibited an ultralow detection limit (32 fM), showed excellent specificity and performed well in serum samples. The sensor was applied to detect miRNA-423-5p in clinical plasma samples from healthy individuals and HF patients, and the results revealed its good clinical application in HF diagnosis. Thus, the developed colorimetric sensor provides a convenient detection tool for early screening and diagnosis of HF, as well as for pathophysiological studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Colorimetría / ADN Catalítico / MicroARNs / Nanopartículas del Metal / Oro Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Colorimetría / ADN Catalítico / MicroARNs / Nanopartículas del Metal / Oro Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos