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Growth on stiffer substrates impacts animal health and longevity in C. elegans.
Oorloff, Maria; Hruby, Adam; Averbukh, Maxim; Alcala, Athena; Dutta, Naibedya; Minor, Cray; Castro Torres, Toni; Moaddeli, Darius; Vega, Matthew; Kim, Juri; Bong, Andrew; Coakley, Aeowynn J; Hicks, Daniel; Wang, Jing; Wang, Tiffany; Hoang, Sally; Tharp, Kevin M; Garcia, Gilberto; Higuchi-Sanabria, Ryo.
Afiliación
  • Oorloff M; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Hruby A; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Averbukh M; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Alcala A; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Dutta N; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Minor C; Cancer Metabolism and Microenvironment Program, Sanford Burnham Prebys, La Jolla, CA, United States of America.
  • Castro Torres T; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Moaddeli D; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Vega M; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Kim J; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Bong A; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Coakley AJ; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Hicks D; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Wang J; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Wang T; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Hoang S; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Tharp KM; Cancer Metabolism and Microenvironment Program, Sanford Burnham Prebys, La Jolla, CA, United States of America.
  • Garcia G; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
  • Higuchi-Sanabria R; Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, United States of America.
PLoS One ; 19(9): e0302673, 2024.
Article en En | MEDLINE | ID: mdl-39264947
ABSTRACT
Mechanical stress is a measure of internal resistance exhibited by a body or material when external forces, such as compression, tension, bending, etc. are applied. The study of mechanical stress on health and aging is a continuously growing field, as major changes to the extracellular matrix and cell-to-cell adhesions can result in dramatic changes to tissue stiffness during aging and diseased conditions. For example, during normal aging, many tissues including the ovaries, skin, blood vessels, and heart exhibit increased stiffness, which can result in a significant reduction in function of that organ. As such, numerous model systems have recently emerged to study the impact of mechanical and physical stress on cell and tissue health, including cell-culture conditions with matrigels and other surfaces that alter substrate stiffness and ex vivo tissue models that can apply stress directly to organs like muscle or tendons. Here, we sought to develop a novel method in an in vivo model organism setting to study the impact of altering substrate stiffness on aging by changing the stiffness of solid agar medium used for growth of C. elegans. We found that greater substrate stiffness had limited effects on cellular health, gene expression, organismal health, stress resilience, and longevity. Overall, our study reveals that altering substrate stiffness of growth medium for C. elegans has only mild impact on animal health and longevity; however, these impacts were not nominal and open up important considerations for C. elegans biologists in standardizing agar medium choice for experimental assays.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caenorhabditis elegans / Longevidad Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caenorhabditis elegans / Longevidad Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos