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A novel pan-proteome array for high-throughput profiling of the humoral response to Treponema pallidum.
Campo, Joseph J; Romeis, Emily; Oberai, Amit; Pablo, Jozelyn V; Hung, Christopher; Teng, Andy A; Shandling, Adam D; Phan, Amber; Haynes, Austin M; Giacani, Lorenzo.
Afiliación
  • Campo JJ; Antigen Discovery, Inc., Irvine, CA, USA.
  • Romeis E; Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA.
  • Oberai A; Antigen Discovery, Inc., Irvine, CA, USA.
  • Pablo JV; Antigen Discovery, Inc., Irvine, CA, USA.
  • Hung C; Antigen Discovery, Inc., Irvine, CA, USA.
  • Teng AA; Antigen Discovery, Inc., Irvine, CA, USA.
  • Shandling AD; Antigen Discovery, Inc., Irvine, CA, USA.
  • Phan A; Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA.
  • Haynes AM; Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA.
  • Giacani L; Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA.
iScience ; 27(9): 110618, 2024 Sep 20.
Article en En | MEDLINE | ID: mdl-39262771
ABSTRACT
Given the resurgence of syphilis, research endeavors to improve current assays for serological diagnosis and management of this disease are a priority. A proteome-scale platform for high-throughput profiling of the humoral response to Treponema pallidum (T. pallidum) proteins during infection could identify antigens suitable to ameliorate the performance and capabilities of treponemal tests for syphilis. Additionally, because infection-induced immunity is partially protective, profiling the response to T. pallidum outer membrane proteins (OMPs) could help select vaccine candidates. Therefore, we developed a pan-proteome array (PPA) based on the Nichols and SS14 strain complete proteomes and used it to define the immunoglobulin M (IgM) and IgG humoral response to T. pallidum proteins in sera collected longitudinally from long-term infected rabbits and from rabbits that were infected, treated, and re-infected. We identified antigens that could facilitate early diagnosis and immunity to a core set of OMP that could explain protection upon reinfection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: IScience Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: IScience Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos