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Mutagenicity of the agriculture pesticide chlorothalonil assessed by somatic mutation and recombination test in Drosophila melanogaster.
Veber, Bruno; do Amaral Flores, Mariana; Lehmann, Mauricio; da Rosa, Carlos Eduardo; Hoff, Mariana Leivas Müller.
Afiliación
  • Veber B; Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG)-Campus Carreiros, Rio Grande, Rio Grande do Sul, Brazil.
  • do Amaral Flores M; Laboratório de Toxicidade Genética - TOXIGEN, Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada à Saúde (PPGBioSaúde), Universidade Luterana do Brasil (ULBRA), Canoas, Rio Grande do Sul, Brazil.
  • Lehmann M; Laboratório de Toxicidade Genética - TOXIGEN, Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada à Saúde (PPGBioSaúde), Universidade Luterana do Brasil (ULBRA), Canoas, Rio Grande do Sul, Brazil.
  • da Rosa CE; Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG)-Campus Carreiros, Rio Grande, Rio Grande do Sul, Brazil.
  • Hoff MLM; Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG)-Campus Carreiros, Rio Grande, Rio Grande do Sul, Brazil.
Environ Mol Mutagen ; 2024 Sep 12.
Article en En | MEDLINE | ID: mdl-39262276
ABSTRACT
Chlorothalonil (CTL) is a pesticide widely used in Brazil, yet its mutagenic potential is not fully determined. Thus, we assessed the mutagenicity of CTL and its bioactivation metabolites using the somatic mutation and recombination test (SMART) in Drosophila melanogaster, by exposing individuals, with basal and high bioactivation capacities (standard and high bioactivation cross offspring, respectively), from third instar larval to early adult fly stages, to CTL-contaminated substrate (0.25, 1, 10 or 20 µM). This substrate served as food and as physical medium. Increased frequency of large single spots in standard cross flies' wings exposed to 0.25 µM indicates that, if CTL is genotoxic, it may affect Drosophila at early life stages. Since the total spot frequency did not change, CTL cannot be considered mutagenic in SMART. The same long-term exposure design was performed to test whether CTL induces oxidative imbalance in flies with basal (wild-type, WT) or high bioactivation (ORR strain) levels. CTL did not alter reactive oxygen species and antioxidant capacity against peroxyl radicals levels in adult flies. However, lipid peroxidation (LPO) levels were increased in WT male flies exposed to 1 µM CTL. SMART and LPO alterations were observed only in flies with basal bioactivation levels, pointing to direct CTL toxicity to DNA and lipids. Survival, emergence and locomotor behavior were not affected, indicating no bias due to lethality, developmental and behavioral impairment. We suggest that, if related to CTL exposure, DNA and lipid damages may be residual damage of earlier life stages of D. melanogaster.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Environ Mol Mutagen Asunto de la revista: BIOLOGIA MOLECULAR / SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Environ Mol Mutagen Asunto de la revista: BIOLOGIA MOLECULAR / SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos