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Quantification of the interaction forces between dengue virus and dopamine type-2 receptor using optical tweezers.
Arifin, Jane C; Tsai, Bo-Ying; Chen, Chun-Yu; Chu, Li-Wei; Lin, Yi-Ling; Lee, Chau-Hwang; Chiou, Arthur; Ping, Yueh-Hsin.
Afiliación
  • Arifin JC; Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
  • Tsai BY; Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
  • Chen CY; Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
  • Chu LW; Department and Institute of Pharmacology, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
  • Lin YL; Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan.
  • Lee CH; Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
  • Chiou A; Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
  • Ping YH; Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
Virol J ; 21(1): 215, 2024 Sep 11.
Article en En | MEDLINE | ID: mdl-39261951
ABSTRACT

BACKGROUND:

Dengue virus (DENV) causes the most significant mosquito-borne viral disease with a wide spectrum of clinical manifestation, including neurological symptoms associated with lethal dengue diseases. Dopamine receptors are expressed in central nervous system, and dopamine antagonists have been reported to exhibit antiviral activity against DENV infection in vivo and in vitro. Although identification of host-cell receptor is critical to understand dengue neuropathogenesis and neurotropism, the involvement of dopamine receptors in DENV infection remains unclear.

RESULTS:

We exploited the sensitivity and precision of force spectroscopy to address whether dopamine type-2 receptors (D2R) directly interact with DENV particles at the first step of infection. Using optical tweezers, we quantified and characterized DENV binding to D2R expressed on Chinese hamster ovary (CHO) cells. Our finding suggested that the binding was D2R- and DENV-dependent, and that the binding force was in the range of 50-60 pN. We showed that dopamine antagonists prochlorperazine (PCZ) and trifluoperazine (TFP), previously reported to inhibit dengue infection, interrupt the DENV-D2R specific binding.

CONCLUSIONS:

This study demonstrates that D2R could specifically recognize DENV particles and function as an attachment factor on cell surfaces for DENV. We propose D2R as a host receptor for DENV and as a potential therapeutic target for anti-DENV drugs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cricetulus / Receptores de Dopamina D2 / Virus del Dengue / Pinzas Ópticas Límite: Animals / Humans Idioma: En Revista: Virol J Asunto de la revista: VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cricetulus / Receptores de Dopamina D2 / Virus del Dengue / Pinzas Ópticas Límite: Animals / Humans Idioma: En Revista: Virol J Asunto de la revista: VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido