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Cut-offs for relapse detection in men with stage I testicular germ cell tumors during active surveillance within a prospective multicentre cohort study using either raw or housekeeper normalized miR-371a-3p serum levels.
Fankhauser, Christian D; Wettstein, Marian S; Christiansen, Ailsa J; Rothermundt, Christian; Cathomas, Richard; Kaufmann, Ernest; Sigg, Silvan; Templeton, Arnoud J; Hirschi-Blickenstorfer, Anita; Lorch, Anja; Gillessen, Silke; Beyer, Joerg; Hermanns, Thomas.
Afiliación
  • Fankhauser CD; Department of Urology, University of Lucerne, Lucerne, Switzerland; Department of Urology, Luzerner Kantonsspital, Lucerne, Switzerland. Electronic address: cdfankhauser@gmail.com.
  • Wettstein MS; Department of Urology, University of Zurich, Zurich, Switzerland.
  • Christiansen AJ; Department of Pathology and Molecular Pathology, University of Zurich, Zurich, Switzerland.
  • Rothermundt C; Department of Medical Oncology and Hematology, Kantonsspital St. Gallen, St. Gallen, Switzerland.
  • Cathomas R; Department of Oncology, Kantonsspital Chur, Chur, Switzerland.
  • Kaufmann E; Department of Urology, University of Lucerne, Lucerne, Switzerland; Department of Urology, Luzerner Kantonsspital, Lucerne, Switzerland.
  • Sigg S; Department of Urology, Luzerner Kantonsspital, Lucerne, Switzerland.
  • Templeton AJ; Department of Oncology, St. Clara Research and Faculty of Medicine, University of Basel, Basel, Switzerland.
  • Hirschi-Blickenstorfer A; Department of Oncology, Onkozentrum Hirslanden, Klinik Hirslanden, Zurich, Switzerland.
  • Lorch A; Department of Oncology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
  • Gillessen S; Department of Medical Oncology and Hematology, Kantonsspital St. Gallen, St. Gallen, Switzerland; EOC Oncology Institute of Southern Switzerland, Bellinzona, Switzerland; Department of Oncology, Universita della Svizzera Italiana, Lugano, Switzerland; Department of Oncology, University of Bern, Bern
  • Beyer J; Department of Medical Oncology, Inselspital, Berne University Hospital, University of Berne, Berne, Switzerland.
  • Hermanns T; Department of Urology, University of Zurich, Zurich, Switzerland; Department of Urology, Zentrum für Urologie Zurich, Zurich, Switzerland.
Urol Oncol ; 2024 Sep 10.
Article en En | MEDLINE | ID: mdl-39261117
ABSTRACT

PURPOSE:

MiR-371a-3p represents a novel liquid biomarker that detects all histologies of germ-cell tumors (GCT) except teratoma. However, it is currently unclear whether miR-371a-3p results obtained directly from RT-PCR (raw Cq) or normalized for housekeeper genes and transformed into the relative quantity (RQ) value should be used and at what cut-off level. The purpose of this research was to evaluate, which values should be used, and a potential cut-off level for relapse-detection to inform subsequent studies. MATERIALS AND

METHODS:

We applied a CE-certified qRT-PCR test to measure miR-371a-3p at each follow-up visit during active surveillance in 34 men with stage I testicular GCT. MiR-371a-3p levels were calculated by the ΔΔ method.

RESULTS:

About 18 Patients had pure seminoma and 16 had mixed or nonseminomatous testicular GCT. Recurrences were detected in 10 patients and were correctly identified by both raw and housekeeper-normalized miR-371a-3p serum levels. The raw Cq, with a cut-off value of <28, resulted in only 1 false positive (3%), whereas RQ, with a cut-off value of >15, produced 6 false positive results (17%). Most of these false positive results normalized in subsequent measurements. The RQ approach detected recurrence in 1 patient 6 months earlier than the raw Cq approach.

CONCLUSION:

Our preliminary data suggest that this CE-certified assay, using previously suggested cut-off values, is a promising method for detecting disease recurrence, provided a confirmatory second test is conducted to identify false positive results. To avoid unnecessary scans or overtreatment, we are currently validating this assay and cut-offs in a prospective cohort study.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Urol Oncol Asunto de la revista: NEOPLASIAS / UROLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Urol Oncol Asunto de la revista: NEOPLASIAS / UROLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos