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Metabolic engineering of Escherichia coli for seleno-methylselenocysteine production.
Yang, Hulin; Wang, ShiZhuo; Zhao, Meiyi; Liao, Yonghong; Wang, Fenghuan; Yin, Xian.
Afiliación
  • Yang H; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China; School of Light Industry, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China.
  • Wang S; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China; School of Light Industry, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China.
  • Zhao M; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China; School of Light Industry, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China.
  • Liao Y; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China; School of Light Industry, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China.
  • Wang F; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China; School of Light Industry, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China.
  • Yin X; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China; School of Light Industry, Beijing Technology and Business University, Fucheng RD 11, Beijing 100048, China. Electronic address: yinxian@btbu.edu.c
J Biotechnol ; 2024 Sep 09.
Article en En | MEDLINE | ID: mdl-39260702
ABSTRACT
Selenium (Se) is an essential trace element for life. Seleno-methylselenocysteine (SeMCys) can serve as a Se supplement with anticarcinogenic activity and can improve cognitive deficits. We engineered Escherichia coli for microbial production of SeMCys. The genes involved in the synthesis of SeMCys were divided into three modules-the SeCys synthesis, methyl donor synthesis and SMT modules-and expressed in plasmids with different copy numbers. The higher copy number of the SeCys synthesis module facilitated SeMCys production. The major routes for SeCys degradation were then modified. Deletion of the cysteine desulfurase gene csdA or sufS improved SeMCys production the most, and the strain that knocked out both genes doubled SeMCys production. The addition of serine in the mid-logarithmic growth phase significantly improved SeMCys synthesis. When the serine synthetic pathway was enhanced, SeMCys production increased by 12.5%. Fed-batch culture for sodium selenite supplementation in the early stationary phase improved SeMCys production to 3.715mg/L. This is the first report of the metabolic engineering of E. coli for the production of SeMCys and provide information on Se metabolism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos