Your browser doesn't support javascript.
loading
New insights into RNA mycoviruses of fungal pathogens causing Fusarium head blight.
Buivydaite, Zivile; Winding, Anne; Jørgensen, Lise Nistrup; Zervas, Athanasios; Sapkota, Rumakanta.
Afiliación
  • Buivydaite Z; Department of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde 4000, Denmark.
  • Winding A; Department of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde 4000, Denmark.
  • Jørgensen LN; Department of Agroecology, Aarhus University, Forsøgsvej 1, Slagelse 4200, Denmark.
  • Zervas A; Department of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde 4000, Denmark.
  • Sapkota R; Department of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde 4000, Denmark. Electronic address: rs@envs.au.dk.
Virus Res ; 349: 199462, 2024 Nov.
Article en En | MEDLINE | ID: mdl-39260572
ABSTRACT
Fusarium head blight (FHB) continues to be a major problem in wheat production and is considered a disease complex caused by several fungal pathogens including Fusarium culmorum, F. graminearum and F. equiseti. With the objective of investigating diversity of mycoviruses in FHB-associated pathogens, we isolated Fusarium spp. from six wheat (Triticum aestivum) cultivars. In total, 56 Fusarium isolates (29 F. culmorum, 24 F. graminearum, one F. equiseti) were screened for mycoviruses by extracting and sequencing double-stranded RNA. We found that a large proportion of Fusarium isolates (46 %) were infected with mycoviruses. F. culmorum, previously described to harbor only one mycovirus, tended to host more viruses than F. graminearum, with a few isolates harboring seven mycoviruses simultaneously. Based on the RNA-dependent RNA polymerase domain analysis, ten were positive-sense single-stranded RNA viruses (related to viruses from families Mitoviridae, Botourmiaviridae, Narnaviridae, Tymoviridae, Gammaflexiviridae, as well as proposed Ambiguiviridae and ormycovirus viral group), one was double-stranded RNA virus (Partitiviridae), and five were negative-sense single-stranded RNA viruses (related to members in the families of Yueviridae, Phenuiviridae, Mymonaviridae, as well as proposed Mycoaspiviridae). Five mycoviruses were shared between F. graminearum and F. culmorum. These results increase our general understanding of mycovirology. To our knowledge, this is the first in-depth report of the mycovirome in F. culmorum and the first report on the diversity of mycoviruses from Danish isolates of FHB-causing fungi in general.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Enfermedades de las Plantas / Virus ARN / Triticum / ARN Viral / Virus Fúngicos / Fusarium Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Enfermedades de las Plantas / Virus ARN / Triticum / ARN Viral / Virus Fúngicos / Fusarium Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Países Bajos