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Aptasensors application for cow's milk allergens detection and early warning: Progress, challenge, and perspective.
Zhang, Qingya; Liu, Ting; Yuan, Xiaomin; Zhao, Xiongjie; Zhou, Liyi.
Afiliación
  • Zhang Q; College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China.
  • Liu T; College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China.
  • Yuan X; College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China.
  • Zhao X; College of Chemistry and Biological Engineering, Hunan University of Science and Engineering, Yongzhou, Hunan, 425199, China. Electronic address: pharmzhxj@huse.edu.cn.
  • Zhou L; College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China. Electronic address: zhouly0817@163.com.
Talanta ; 281: 126808, 2025 Jan 01.
Article en En | MEDLINE | ID: mdl-39260252
ABSTRACT
Cow's milk allergy (CMA) is considered one of the most prevalent food allergies and a public health concern. Modern medical research shows that the effective way to prevent allergic reactions is to prevent allergic patients from consuming allergenic substances. Therefore, the development of rapid and accurate detection technology for milk allergens detection and early warning is critical to safeguarding those with a cow milk allergy. As the oligonucleotide sequences with high specificity and selectivity, aptamers frequently assemble with transduction elements forming multifarious aptasensors for quantitative detection owing to their high-affinity binding to the target. Current aptasensors in the field of cow's milk allergen detection in recent years are explored in this review. This review takes a look back at a few common assays, including ELISA and PCR, before presenting a clear overview of the aptamer and threshold doses. It delves into a detailed discussion of the current aptamer-based detection techniques and related theories for milk allergen identification. Last but not least, we conclude with a discussion and outlook of the advancements made in allergen detection with aptamers. We sincerely hope that there will be more extensive applications for aptasensors in the future contributing to reducing the possibility of patients suffering from adverse reactions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alérgenos / Técnicas Biosensibles / Hipersensibilidad a la Leche / Leche / Aptámeros de Nucleótidos Límite: Animals / Humans Idioma: En Revista: Talanta Año: 2025 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alérgenos / Técnicas Biosensibles / Hipersensibilidad a la Leche / Leche / Aptámeros de Nucleótidos Límite: Animals / Humans Idioma: En Revista: Talanta Año: 2025 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos