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B chromosome and its non-Mendelian inheritance in Atractylodes lancea.
Hara, Kazuya; Kikuchi, Shinji; Inoue, Misaki; Tsusaka, Takahiro; Sakurai, Miki; Tanabe, Hideyuki; Shirasawa, Kenta; Isobe, Sachiko.
Afiliación
  • Hara K; Graduate School of Horticulture, Chiba University, Chiba, Japan.
  • Kikuchi S; Graduate School of Horticulture, Chiba University, Chiba, Japan.
  • Inoue M; Plant Molecular Science Center, Chiba University, Chiba, Japan.
  • Tsusaka T; Research Center for Space Agriculture and Horticulture, Chiba University, Chiba, Japan.
  • Sakurai M; Graduate School of Horticulture, Chiba University, Chiba, Japan.
  • Tanabe H; Botanical Raw Materials Division, Tsumura & Co., Ibaraki, Japan.
  • Shirasawa K; Botanical Raw Materials Division, Tsumura & Co., Ibaraki, Japan.
  • Isobe S; Research Center for Integrative Evolutionary Science, SOKENDAI, Kanagawa, Japan.
PLoS One ; 19(9): e0308881, 2024.
Article en En | MEDLINE | ID: mdl-39259755
ABSTRACT
Supernumerary B chromosomes contribute to intraspecific karyotypic variation. B chromosomes have been detected in more than 2000 organisms; they possess unique and diverse features, including non-Mendelian inheritance. Here, we report one or more B chromosomes in the gynodioecious plant Atractylodes lancea. Among 54 A. lancea lines, 0-2 B chromosomes were detected in both hermaphroditic and female plants, with the B chromosomes appearing as DAPI-bright regions within the nuclei. Genomic in situ hybridization revealed that the B chromosomes had no conserved A chromosome DNA sequences, confirmed by fluorescence in situ hybridization probed with independently dissected B chromosomes. In male meiosis, the B chromosome did not pair with an A chromosome and was therefore eliminated; accordingly, only 20.1% and 18.6% of these univalent B chromosomes remained at the end of meiosis for the 1B lines of KY17-148 and KY17-118, respectively. However, we also found that B chromosomes were transmitted from male parents in 40.8%-44.2% and 47.2% of the next generation; although these transmission rates from male parents were not essentially different from Mendelian inheritance (0.5), the transmission of gametes carrying B chromosomes increased through fertilization or seed development. B chromosomes were transmitted from three of four 1B female parents to 64.3%-92.6% of the next generation, suggesting B chromosome accumulation. We propose that the B chromosome of A. lancea has a specific sequence and persists via non-Mendelian inheritance from female parents. Overall, A. lancea, with its unique characteristics, is a promising model for understanding the structure, evolution, and mechanism of non-Mendelian inheritance of B chromosomes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hibridación Fluorescente in Situ / Cromosomas de las Plantas / Atractylodes / Meiosis Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hibridación Fluorescente in Situ / Cromosomas de las Plantas / Atractylodes / Meiosis Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos