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Widespread geographic distribution of Aedes aegypti (Diptera: Culicidae) kdr variants in Panama.
García, Joel; Chong, Mabelle; Rojas, Ambar L; McMillan, W Owen; Bennett, Kelly L; Lenhart, Audrey E; Chaves, Luis F; Loaiza, Jose R.
Afiliación
  • García J; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, Panama.
  • Chong M; Programa Centroamericano de Maestría en Entomología, Universidad de Panama, Campus Octavio Méndez Pereira, Avenida Transístmica, Panama City, Panama.
  • Rojas AL; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, Panama.
  • McMillan WO; Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City, Panama.
  • Bennett KL; Naos Marine Laboratories, Smithsonian Tropical Research Institute, Panama City, Panama.
  • Lenhart AE; Genomic Surveillance Unit, Wellcome Sanger Institute, Cambridge, UK.
  • Chaves LF; Entomology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.
  • Loaiza JR; Department of Environmental and Occupational Health, School of Public Health, Indiana University, Bloomington, IN, USA.
J Med Entomol ; 2024 Sep 11.
Article en En | MEDLINE | ID: mdl-39259661
ABSTRACT
We searched for evidence of knockdown resistance (kdr) mutations in the voltage-gated sodium channel gene of Aedes aegypti (Linnaeus) (Diptera Culicidae) and Aedes albopictus (Skuse) (Diptera Culicidae) mosquitoes from Panama. Conventional PCR was performed on 469 Ae. aegypti and 349 Ae. albopictus. We did not discover kdr mutations in Ae. albopictus, but 2 nonsynonymous kdr mutations, V1016I (found in 101 mosquitoes) and F1534C (found in 29 of the mosquitoes with the V1016I), were detected in Ae. aegypti. These kdr mutations were present in all specimens that were successfully sequenced for both IIS5-S6 and IIIS6 regions, which included samples collected from 8 of the 10 provinces of Panama. No other kdr mutations were found in Ae. aegypti, including V1016G, which has already been reported in Panama. Findings suggest that the V1016I-F1534C variant is prevalent in Panama, which might be related to the introduction and passive movement of mosquitoes as part of the used-tire trade. However, we cannot rule out the possibility that selection on de novo replacement of kdr mutations also partially explains the widespread distribution pattern of these mutations. These 2 ecological and evolutionary processes are not mutually exclusive, though, as they can occur in tandem. Research in Panama needs to calculate the genotypic and allelic frequencies of kdr alleles in local Ae. aegypti populations and to test whether some combinations confer phenotypic resistance or not. Finally, future studies will have to track the introduction and spreading of new kdr mutations in both Aedes species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE País/Región como asunto: America central / Panama Idioma: En Revista: J Med Entomol Año: 2024 Tipo del documento: Article País de afiliación: Panamá Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE País/Región como asunto: America central / Panama Idioma: En Revista: J Med Entomol Año: 2024 Tipo del documento: Article País de afiliación: Panamá Pais de publicación: Reino Unido