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Histone H3K9 Methylation Features under Hypoxic Conditions after the HIF1A Knockdown in Mesenchymal Stromal Cells In Vitro.
Bobyleva, P I; Tyrina, E A; Lobanova, M V; Buravkova, L B.
Afiliación
  • Bobyleva PI; Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia. blastoblast@gmail.com.
  • Tyrina EA; Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia.
  • Lobanova MV; Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia.
  • Buravkova LB; Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia.
Bull Exp Biol Med ; 177(4): 431-435, 2024 Aug.
Article en En | MEDLINE | ID: mdl-39259464
ABSTRACT
The effects of HIF1A knockdown by RNA interference on the histone H3K9 methylation in human umbilical cord mesenchymal stromal cells in vitro under conditions of 24-h exposure to hypoxia (1% O2) were studied. Evaluation of transcriptional activity of genes involved in the regulation of H3K9 methylation (KDM3A, KDM4A, and EHMT2) and the cytofluorimetric analysis of the expression of the corresponding antigens and H3K9 methylation level demonstrated a pronounced stimulating effect of hypoxic exposure. Moreover, the expression of KDM4A and EHMT2 was regulated by HIF1A-mediated mechanism, unlike KDM3A; the level of the corresponding proteins depended on HIF1A. In addition, the HIF-1-dependent regulation of KDM3A, KDM4A, and EHMT2/G9a, and directly the H3K9 methylation level in mesenchymal stromal cells also took place under normoxia conditions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Histonas / Hipoxia de la Célula / Subunidad alfa del Factor 1 Inducible por Hipoxia / Histona Demetilasas con Dominio de Jumonji / Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Bull Exp Biol Med Año: 2024 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Histonas / Hipoxia de la Célula / Subunidad alfa del Factor 1 Inducible por Hipoxia / Histona Demetilasas con Dominio de Jumonji / Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Bull Exp Biol Med Año: 2024 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos