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CD146 Molecule Expression in B Cells Acute Lymphoblastic Leukemia (B-ALLs): A Flow-Cytometric Marker for an Accurate Diagnostic Workup.
Laganà, Alessandro; Totaro, Matteo; Bisegna, Maria Laura; Elia, Loredana; Intoppa, Stefania; Beldinanzi, Marco; Matarazzo, Mabel; di Trani, Mariangela; Costa, Alessandro; Maglione, Raffaele; Mandelli, Biancamaria; Chiaretti, Sabina; Martelli, Maurizio; De Propris, Maria Stefania.
Afiliación
  • Laganà A; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Totaro M; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Bisegna ML; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Elia L; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Intoppa S; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Beldinanzi M; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Matarazzo M; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • di Trani M; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Costa A; Hematology Unit, Businco Hospital, Department of Medical Sciences and Public Health, University of Cagliari, Cagliari, Italy.
  • Maglione R; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Mandelli B; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Chiaretti S; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • Martelli M; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
  • De Propris MS; Hematology, Department of Translational and Precision Medicine, Sapienza University, 00161 Rome, Italy.
Mediterr J Hematol Infect Dis ; 16(1): e2024064, 2024.
Article en En | MEDLINE | ID: mdl-39258185
ABSTRACT

Background:

B-lineage acute lymphoblastic leukemias (B-ALL) harboring the t(9;22)(q34;q11)/BCRABL1 rearrangement represent a category with previously dismal prognosis whose management and outcome dramatically changed thanks to the use of tyrosine kinase inhibitors (TKIs) usage and more recently full chemo-free approaches. The prompt identification of these cases represents an important clinical need.

Objectives:

We sought to identify an optimized cytofluorimetric diagnostic panel to predict the presence of Philadelphia chromosome (Ph) in B-ALL cases by the introduction of CD146 in our multiparametric flow cytometry (MFC) panels.

Methods:

We prospectively evaluated a total of 245 cases of newly diagnosed B-ALLs with a CD146 positivity threshold >10% referred to the Division of Hematology of 'Sapienza' University of Rome. We compared the results of CD146 expression percentage and its mean fluorescence intensity (MFI) between Ph+ ALLs, Ph-like ALLs, and molecularly negative ALLs.

Results:

Seventy-nine of the 245 B-ALL cases (32%) did not present mutations at molecular testing, with 144/245 (59%) resulting in Ph+ ALL and 19/245 (8%) Ph-like ALLs. Comparing the 3 groups, we found that Ph+ B-ALLs were characterized by higher expression percentage of myeloid markers such as CD13, CD33, and CD66c and low expression of CD38; Ph+ B-ALL showed a higher CD146 expression percentage and MFI when compared with both molecular negative B-ALL and Ph-like ALLs; neither the mean percentage of CD146 expression neither CD146 MFI were statically different between molecular negative B-ALL and Ph-like ALLs.

Conclusions:

Our data demonstrate the association between CD146 expression and Ph+ ALLs. CD146, along with myeloid markers, may help to identify a distinctive immunophenotypic pattern, useful for rapid identification in the diagnostic routine of this subtype of B-ALLs that benefits from a specific therapeutic approach.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mediterr J Hematol Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Italia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mediterr J Hematol Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Italia