Migration and retention of human osteosarcoma cells in bioceramic graft with open channel architecture designed for bone tissue engineering.
Biomed Mater
; 19(6)2024 Sep 18.
Article
en En
| MEDLINE
| ID: mdl-39255821
ABSTRACT
The microstructure of a porous bioceramic bone graft, especially the pore architecture, plays a crucial role in the performance of the graft. Conventional bioceramic grafts typically feature a random, closed-pore structure, limiting biological activity to the periphery of the graft. This can lead to delay in full integration with the host site. Bioceramic forms with open through pores can perform better because their inner regions are accessible for natural bone remodeling. This study explores the influence of open through pores in a bioceramic graft on the migration and retention of the local cellsin vitro, which will correlate to the rate of healingin vivo.Hydroxyapatite ceramic forms with aligned channels were fabricated using slip casting technique, employing sacrificial fibers. The sorption characteristics across the graft were evaluated using human osteosarcoma cell line. Seven-day cultures showed viable cells within the channels, confirmed by live/dead assay, scanning electron microscope analysis, and cytoskeletal staining, indicating successful cell colonization. The channel architecture effectively enhances cell migration and retention throughout its entire structure, suggesting potential applications in bone tissue engineering based on the results obtained.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteosarcoma
/
Movimiento Celular
/
Cerámica
/
Durapatita
/
Ingeniería de Tejidos
/
Andamios del Tejido
Límite:
Humans
Idioma:
En
Revista:
Biomed Mater
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Reino Unido