Selective IgG binding to the LPS glycolipid core found in bovine colostrum, or milk, during <i>Escherichia coli</i> mastitis influences endotoxin function.

Hurst, Suzanne M; Flossdorf, David A L; Koralagamage Don, Raveen; Pernthaner, Anton

Selective IgG binding to the LPS glycolipid core found in bovine colostrum, or milk, during Escherichia coli mastitis influences endotoxin function.

Hurst, Suzanne M; Flossdorf, David A L; Koralagamage Don, Raveen; Pernthaner, Anton.

Afiliación

Hurst SM; Koru Diagnostics Ltd, Estendart Research Centre, Aviation Way, Massey University, Palmerston North, New Zealand.
Flossdorf DAL; Animal Breeding and Genomics, Wageningen University and Research, Wageningen, Netherlands.
Koralagamage Don R; Koru Diagnostics Ltd, Estendart Research Centre, Aviation Way, Massey University, Palmerston North, New Zealand.
Pernthaner A; Koru Diagnostics Ltd, Estendart Research Centre, Aviation Way, Massey University, Palmerston North, New Zealand.

Innate Immun ; 30(5): 96-118, 2024 Jul.

Article en En | MEDLINE | ID: mdl-39252173

ABSTRACT

ABSTRACT

The dynamic interplay between intramammary IgG, formation of antigen-IgG complexes and effector immune cell function is essential for immune homeostasis within the bovine mammary gland. We explore how changes in the recognition and binding of anti-LPS IgG to the glycolipid "functional" core in milk from healthy or clinically diagnosed Escherichia coli (E. coli) mastitis cows' controls endotoxin function. In colostrum, we found a varied anti-LPS IgG repertoire and novel soluble LPS/IgG complexes with direct IgG binding to the LPS glycolipid core. These soluble complexes, absent in milk from healthy lactating cows, were evident in cows diagnosed with E. coli mastitis and correlated with endotoxin-driven inflammation. E. coli mastitis milk displayed a proportional reduction in anti-LPS glycolipid core IgG compared to colostrum. Milk IgG extracts showed that only colostrum IgG attenuated LPS induced endotoxin activity. Furthermore, LPS-stimulated reactive oxygen species (ROS) in milk granulocytes was only suppressed by colostrum IgG, while IgG extracts of neither colostrum nor E. coli mastitis milk influenced N-formylmethionine-leucyl-phenylalanine (fMLP)-stimulated ROS in LPS primed granulocytes. Our findings support bovine intramammary IgG diversity in health and in response to E. coli infection generate milk anti-LPS IgG repertoires that coordinate appropriate LPS innate-adaptive immune responses essential for animal health.

Asunto(s)

Calostro; Infecciones por Escherichia coli; Escherichia coli; Glucolípidos; Inmunoglobulina G; Lipopolisacáridos; Mastitis Bovina; Leche; Animales; Bovinos; Femenino; Calostro/inmunología; Calostro/metabolismo; Inmunoglobulina G/inmunología; Inmunoglobulina G/metabolismo; Mastitis Bovina/inmunología; Mastitis Bovina/microbiología; Escherichia coli/inmunología; Lipopolisacáridos/inmunología; Leche/inmunología; Glucolípidos/metabolismo; Glucolípidos/inmunología; Infecciones por Escherichia coli/inmunología; Endotoxinas/inmunología; Endotoxinas/metabolismo; Especies Reactivas de Oxígeno/metabolismo; Granulocitos/inmunología; Granulocitos/metabolismo; Unión Proteica; Glándulas Mamarias Animales/inmunología; Glándulas Mamarias Animales/metabolismo

Palabras clave

Anti-LPS IgG; E. coli mastitis; LPS/IgG complexes; bovine colostrum; endotoxin function

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina G / Glucolípidos / Lipopolisacáridos / Calostro / Leche / Escherichia coli / Infecciones por Escherichia coli / Mastitis Bovina Límite: Animals Idioma: En Revista: Innate Immun Asunto de la revista: ALERGIA E IMUNOLOGIA / BACTERIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Estados Unidos

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