Your browser doesn't support javascript.
loading
Rejuvenation of aged oocyte through exposure to young follicular microenvironment.
Wang, HaiYang; Huang, Zhongwei; Shen, Xingyu; Lee, Yaelim; Song, XinJie; Shu, Chang; Wu, Lik Hang; Pakkiri, Leroy Sivappiragasam; Lim, Poh Leong; Zhang, Xi; Drum, Chester Lee; Zhu, Jin; Li, Rong.
Afiliación
  • Wang H; Mechanobiology Institute, National University of Singapore, Singapore, Singapore. mbihw@nus.edu.sg.
  • Huang Z; NUS Bia Echo Asia Centre for Reproductive Longevity and Equality, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
  • Shen X; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
  • Lee Y; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
  • Song X; Mechanobiology Institute, National University of Singapore, Singapore, Singapore.
  • Shu C; Mechanobiology Institute, National University of Singapore, Singapore, Singapore.
  • Wu LH; Mechanobiology Institute, National University of Singapore, Singapore, Singapore.
  • Pakkiri LS; Mechanobiology Institute, National University of Singapore, Singapore, Singapore.
  • Lim PL; Cardiovascular Research Institute, National University Health System, Singapore, Singapore.
  • Zhang X; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
  • Drum CL; Department of Pharmacy, Faculty of Science, National University of Singapore, Singapore, Singapore.
  • Zhu J; Cardiovascular Research Institute, National University Health System, Singapore, Singapore.
  • Li R; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
Nat Aging ; 4(9): 1194-1210, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39251866
ABSTRACT
Reproductive aging is a major cause of fertility decline, attributed to decreased oocyte quantity and developmental potential. A possible cause is aging of the surrounding follicular somatic cells that support oocyte growth and development by providing nutrients and regulatory factors. Here, by creating chimeric follicles, whereby an oocyte from one follicle was transplanted into and cultured within another follicle whose native oocyte was removed, we show that young oocytes cultured in aged follicles exhibited impeded meiotic maturation and developmental potential, whereas aged oocytes cultured within young follicles were significantly improved in rates of maturation, blastocyst formation and live birth after in vitro fertilization and embryo implantation. This rejuvenation of aged oocytes was associated with enhanced interaction with somatic cells, transcriptomic and metabolomic remodeling, improved mitochondrial function and higher fidelity of meiotic chromosome segregation. These findings provide the basis for a future follicular somatic cell-based therapy to treat female infertility.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Rejuvenecimiento / Folículo Ovárico Límite: Animals Idioma: En Revista: Nat Aging Año: 2024 Tipo del documento: Article País de afiliación: Singapur Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Rejuvenecimiento / Folículo Ovárico Límite: Animals Idioma: En Revista: Nat Aging Año: 2024 Tipo del documento: Article País de afiliación: Singapur Pais de publicación: Estados Unidos