Your browser doesn't support javascript.
loading
A comprehensive study of CYP2E1 and its role in carcass characteristics and chemical lamb meat quality in different Indonesian sheep breeds.
Harahap, Ratna Sholatia; Gunawan, Asep; Endrawati, Yuni Cahya; Darusman, Huda Shalahudin; Andersson, Göran; Noor, Ronny Rachman.
Afiliación
  • Harahap RS; Faculty of Animal Science, Post-Doctoral Animal Production and Technology Student, IPB University, Bogor, Indonesia.
  • Gunawan A; Faculty of Animal Science, Jambi University, Jambi, Indonesia.
  • Endrawati YC; Faculty of Animal Science, Department of Animal Production and Technology, IPB University, Bogor, Indonesia.
  • Darusman HS; Faculty of Animal Science, Department of Animal Production and Technology, IPB University, Bogor, Indonesia.
  • Andersson G; Department of Anatomy, Physiology and Pharmacology, School of Veterinary and Biomedical Sciences, IPB University, Bogor, Indonesia.
  • Noor RR; Primate Research Centre, Institute of Research and Community Service IPB University, Bogor, Indonesia.
PLoS One ; 19(9): e0310336, 2024.
Article en En | MEDLINE | ID: mdl-39250496
ABSTRACT
The role of CYP2E1 in oxidation is essential for its effects on meat quality. This study used 200 Indonesian sheep (Ovis aries) to determine the SNP g allele frequencies. g. 50658168 T>C of CYP2E1 gene located in 3´-UTR region and their genetic association with lamb quality traits, including carcass characteristics, retail cut carcass, physicochemical lamb, fatty acid, cholesterol, flavor and odor, and mineral content. Further, the level of CYP2E1 mRNA and CYP2E1 protein expression in muscle were determined and correlated with lamb quality traits. CYP2E1 gene polymorphisms were identified using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The CYP2E1 mRNA expression levels in phenotypically divergent sheep populations were analyzed using Quantitative Real Time-PCR (qRT-PCR). Immunohistochemistry (IHC) and hematoxylin-eosin (HE) staining analysis used three samples each in the high and low lamb quality groups based on pH value and tenderness. An association study of CYP2E1 gene polymorphisms was performed using General Linear Model (GLM) analysis. The genetic association between the CC, CT, and TT genotypes at the SNP g. 50658168 T>C CYP2E1 gene and lamb quality traits were significant (P<0.05), including carcass characteristics, retail cut carcass, fatty acid, cholesterol, flavor, and odor. Lambs with the CT genotype had a higher mRNA and protein expression in high lamb quality traits. The highest CYP2E1 protein expression was localized in the longissimus dorsi. The group sample with high lamb quality had a higher area and perimeter of muscle cells. CYP2E1 can be used as a genetic marker for selecting sheep with high meat quality.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citocromo P-450 CYP2E1 / Polimorfismo de Nucleótido Simple Límite: Animals País/Región como asunto: Asia Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citocromo P-450 CYP2E1 / Polimorfismo de Nucleótido Simple Límite: Animals País/Región como asunto: Asia Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Estados Unidos