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Integrated Electrochemical Aptamer Biosensing and Colorimetric pH Monitoring via Hydrogel Microneedle Assays for Assessing Antibiotic Treatment.
Keyvani, Fatemeh; GhavamiNejad, Peyman; Saleh, Mahmoud Ayman; Soltani, Mohammad; Zhao, Yusheng; Sadeghzadeh, Sadegh; Shakeri, Arash; Chelle, Pierre; Zheng, Hanjia; Rahman, Fasih A; Mahshid, Sarah; Quadrilatero, Joe; Rao, Praveen P N; Edginton, Andrea; Poudineh, Mahla.
Afiliación
  • Keyvani F; Department of Electrical and Computer Engineering, Faculty of Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • GhavamiNejad P; Department of Electrical and Computer Engineering, Faculty of Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Saleh MA; Department of Bioengineering, McGill University, 815 Sherbrooke St. W, Montreal, Quebec, H3A 0C3, Canada.
  • Soltani M; Department of Electrical and Computer Engineering, Faculty of Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Zhao Y; School of Pharmacy, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Sadeghzadeh S; Department of Electrical and Computer Engineering, Faculty of Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Shakeri A; School of Pharmacy, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Chelle P; School of Pharmacy, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Zheng H; Department of Electrical and Computer Engineering, Faculty of Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Rahman FA; Department of Kinesiology and Health Sciences, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Mahshid S; Department of Bioengineering, McGill University, 815 Sherbrooke St. W, Montreal, Quebec, H3A 0C3, Canada.
  • Quadrilatero J; Department of Kinesiology and Health Sciences, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Rao PPN; School of Pharmacy, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Edginton A; School of Pharmacy, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
  • Poudineh M; Department of Electrical and Computer Engineering, Faculty of Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
Adv Sci (Weinh) ; : e2309027, 2024 Sep 09.
Article en En | MEDLINE | ID: mdl-39250329
ABSTRACT
Current methods for therapeutic drug monitoring (TDM) have a long turnaround time as they involve collecting patients' blood samples followed by transferring the samples to medical laboratories where sample processing and analysis are performed. To enable real-time and minimally invasive TDM, a microneedle (MN) biosensor to monitor the levels of two important antibiotics, vancomycin (VAN) and gentamicin (GEN) is developed. The MN biosensor is composed of a hydrogel MN (HMN), and an aptamer-functionalized flexible (Flex) electrode, named HMN-Flex. The HMN extracts dermal interstitial fluid (ISF) and transfers it to the Flex electrode where sensing of the target antibiotics happens. The HMN-Flex performance is validated ex vivo using skin models as well as in vivo in live rat animal models. Data is leveraged from the HMN-Flex system to construct pharmacokinetic profiles for VAN and GEN and compare these profiles with conventional blood-based measurements. Additionally, to track pH and monitor patient's response during antibiotic treatment, an HMN is developed that employs a colorimetric method to detect changes in the pH, named HMN-pH assay, whose performance has been validated both in vitro and in vivo. Further, multiplexed antibiotic and pH detection is achieved by simultaneously employing the HMN-pH and HMN-Flex on live animals.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Adv Sci (Weinh) Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Adv Sci (Weinh) Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Alemania