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Sonothrombolysis Using Microfluidically Produced Microbubbles in a Murine Model of Deep Vein Thrombosis.
Xie, Yanjun; Huang, Yi; Stevenson, Hugo C S; Yin, Li; Zhang, Kaijie; Islam, Zain Husain; Marcum, William Aaron; Johnston, Campbell; Hoyt, Nicholas; Kent, Eric William; Wang, Bowen; Hossack, John A.
Afiliación
  • Xie Y; Department of Biomedical Engineering, University of Virginia, 415 Lane Road, Charlottesville, VA, 22908, USA.
  • Huang Y; Department of Biomedical Engineering, University of Virginia, 415 Lane Road, Charlottesville, VA, 22908, USA.
  • Stevenson HCS; Department of Biomedical Engineering, University of Virginia, 415 Lane Road, Charlottesville, VA, 22908, USA.
  • Yin L; Department of Surgery, School of Medicine, University of Virginia, 409 Lane Rd MR4, Charlottesville, VA, 22908, USA.
  • Zhang K; Feinberg School of Medicine, Northwestern University, 300 E. Superior St. Tarry Building, Chicago, IL, 60611, USA.
  • Islam ZH; Department of Surgery, School of Medicine, University of Virginia, 409 Lane Rd MR4, Charlottesville, VA, 22908, USA.
  • Marcum WA; Feinberg School of Medicine, Northwestern University, 300 E. Superior St. Tarry Building, Chicago, IL, 60611, USA.
  • Johnston C; Department of Surgery, School of Medicine, University of Virginia, 409 Lane Rd MR4, Charlottesville, VA, 22908, USA.
  • Hoyt N; Department of Surgery, School of Medicine, University of Virginia, 409 Lane Rd MR4, Charlottesville, VA, 22908, USA.
  • Kent EW; Department of Surgery, School of Medicine, University of Virginia, 409 Lane Rd MR4, Charlottesville, VA, 22908, USA.
  • Wang B; Department of Surgery, School of Medicine, University of Virginia, 409 Lane Rd MR4, Charlottesville, VA, 22908, USA.
  • Hossack JA; Department of Surgery, School of Medicine, University of Virginia, 409 Lane Rd MR4, Charlottesville, VA, 22908, USA.
Ann Biomed Eng ; 2024 Sep 09.
Article en En | MEDLINE | ID: mdl-39249170
ABSTRACT
The need for safe and effective methods to manage deep vein thrombosis (DVT), given the risks associated with anticoagulants and thrombolytic agents, motivated research into innovative approaches to resolve blood clots. In response to this challenge, sonothrombolysis is being explored as a technique that combines microbubbles, ultrasound, and thrombolytic agents to facilitate the aggressive dissolution of thrombi. Prior studies have indicated that relatively large microbubbles accelerate the dissolution process, either in an in vitro or an arterial model. However, sonothrombolysis using large microbubbles must be evaluated in venous thromboembolism diseases, where blood flow velocity is not comparable. In this study, the efficacy of sonothrombolysis was validated in a murine model of pre-existing DVT. During therapy, microfluidically produced microbubbles of 18 µm diameter and recombinant tissue plasminogen activator (rt-PA) were administered through a tail vein catheter for 30 min, while ultrasound was applied to the abdominal region of the mice. Three-dimensional ultrasound scans were performed before and after therapy for quantification. The residual volume of the thrombi was 20% in animals post sonothrombolysis versus 52% without therapy ( p = 0.012 < 0.05 ), indicating a significant reduction in DVT volume. Histological analysis of tissue sections confirmed a reduction in DVT volume post-therapy. Therefore, large microbubbles generated from a microfluidic device show promise in ultrasound-assisted therapy to address concerns related to venous thromboembolism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Ann Biomed Eng Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Ann Biomed Eng Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos