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Enhancing cellular and enzymatic properties through in vivo continuous evolution.
Chu, Weiran; Guo, Yaxin; Wu, Yaokang; Lv, Xueqin; Li, Jianghua; Liu, Long; Du, Guocheng; Chen, Jian; Liu, Yanfeng.
Afiliación
  • Chu W; Jiangnan University, School of Biotechnology, CHINA.
  • Guo Y; Jiangnan University, School of Biotechnology, CHINA.
  • Wu Y; Jiangnan University, School of Biotechnology, CHINA.
  • Lv X; Jiangnan University, School of Biotechnology, CHINA.
  • Li J; Jiangnan University, School of Biotechnology, CHINA.
  • Liu L; Jiangnan University, School of Biotechnology, CHINA.
  • Du G; Jiangnan University, School of Biotechnology, CHINA.
  • Chen J; Jiangnan University, School of Biotechnology, CHINA.
  • Liu Y; Jiangnan University, School of Biotechnology, No. 1800, Lihu Avenue, 214122, Wuxi, CHINA.
Chembiochem ; : e202400564, 2024 Sep 09.
Article en En | MEDLINE | ID: mdl-39248206
ABSTRACT
Directed evolution seeks to evolve target genes at a rate far exceeding the natural mutation rate, thereby endowing cellular and enzymatic properties with desired traits. In vivo continuous directed evolution achieves these purposes by generating libraries within living cells, enabling a continuous cycle of mutant generation and selection, enhancing the exploration of gene variants. Continuous evolution has become powerful tools for unraveling evolution mechanism and improving cellular and enzymatic properties. This review categorizes current continuous evolution into three distinct classes non-targeted chromosomal, targeted chromosomal, and extra-chromosomal hypermutation approaches. It also compares various continuous evolution strategies based on different principles, providing a reference for selecting suitable methods for specific evolutionary goals. Furthermore, this review discusses the two primary limitations for further widespread application of in vivo continuous evolution, which are lack of general applicability and insufficient mutagenic capability. We envision that developing generally applicable mutagenic components and methods to enhance mutation rates for in vivo continuous evolution are promising future directions for wide range applications of continuous evolution.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania