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Genotypic and phenotypic characterisation of asymptomatic bacteriuria (ABU) isolates displaying bacterial interference against multi-drug resistant uropathogenic E. Coli.
Kenneally, Ciara; Murphy, Craig P; Sleator, Roy D; Culligan, Eamonn P.
Afiliación
  • Kenneally C; Department of Biological Sciences, Munster Technological University, Cork, T12 P928, Bishopstown, Ireland.
  • Murphy CP; Department of Biological Sciences, Munster Technological University, Cork, T12 P928, Bishopstown, Ireland.
  • Sleator RD; Department of Biological Sciences, Munster Technological University, Cork, T12 P928, Bishopstown, Ireland.
  • Culligan EP; Department of Biological Sciences, Munster Technological University, Cork, T12 P928, Bishopstown, Ireland. eamonn.culligan@mtu.ie.
Arch Microbiol ; 206(10): 394, 2024 Sep 09.
Article en En | MEDLINE | ID: mdl-39245770
ABSTRACT
Escherichia coli can colonise the urogenital tract of individuals without causing symptoms of infection, in a condition referred to as asymptomatic bacteriuria (ABU). ABU isolates can protect the host against symptomatic urinary tract infections (UTIs) by bacterial interference against uropathogenic E. coli (UPEC). The aim of this study was to investigate the genotypic and phenotypic characteristics of five ABU isolates from midstream urine samples of adults. Comparative genomic and phenotypic analysis was conducted including an antibiotic resistance profile, pangenome analysis, and a putative virulence profile. Based on the genome analysis, the isolates consisted of one from phylogroup A, three from phylogroup B2, and one from phylogroup D. Two of the isolates, PUTS 58 and SK-106-1, were noted for their lack of antibiotic resistance and virulence genes compared to the prototypic ABU strain E. coli 83,972. This study provides insights into the genotypic and phenotypic profiles of uncharacterised ABU isolates, and how relevant fitness and virulence traits can impact their potential suitability for therapeutic bacterial interference.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Bacteriuria / Infecciones Urinarias / Farmacorresistencia Bacteriana Múltiple / Infecciones por Escherichia coli / Escherichia coli Uropatógena / Genotipo / Antibacterianos Límite: Adult / Humans Idioma: En Revista: Arch Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Bacteriuria / Infecciones Urinarias / Farmacorresistencia Bacteriana Múltiple / Infecciones por Escherichia coli / Escherichia coli Uropatógena / Genotipo / Antibacterianos Límite: Adult / Humans Idioma: En Revista: Arch Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Alemania