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Polyol-assisted ternary deep eutectic solvent protective lignocellulose pretreatment for high-efficiency xylan utilization and ethanol production.
Li, Shan; Wang, Yang; Dong, Qinghe; Yuan, Zitong; Mu, Tiancheng; Xue, Zhimin; Cao, Limin.
Afiliación
  • Li S; Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China.
  • Wang Y; Beijing Key Laboratory of Lignocellulosic Chemistry, College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China.
  • Dong Q; Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China.
  • Yuan Z; Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China.
  • Mu T; School of chemistry and life resources, Renmin University of China, Beijing 100872, China.
  • Xue Z; Beijing Key Laboratory of Lignocellulosic Chemistry, College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: zmxue@bjfu.edu.cn.
  • Cao L; Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China. Electronic address: caolimin@cnu.edu.cn.
Carbohydr Polym ; 346: 122628, 2024 Dec 15.
Article en En | MEDLINE | ID: mdl-39245529
ABSTRACT
The traditional lignocellulose pretreatment by deep eutectic solvent (DES) was usually conducted under higher acidic, alkaline and high temperature conditions, which leads to the severe degradation of xylan, decreasing the subsequent reducing sugar concentration by enzymatic hydrolysis and further ethanol fermentation. It is essential to develop an effective DES that selectively removes lignin while preventing excessive xylan degradation during lignocellulose pretreatment. An effective ethylene glycol-assisted ternary DES was designed to treat corn straw (CS) at 100 °C for 6 h. 65.51 % lignin removal was achieved, over 93.46 % cellulose and 50.22 % xylan were retained in pretreated CS with excellent enzymatic digestibility (glucan conversion of 77.05 % and xylan conversion of 71.72 %), total sugar conversion could reach 75.93 %, implying the unique capacity to selectively remove lignin while preserving carbohydrate components. Furthermore, the universality of the selective removal of lignin and effective retention of xylan by ternary DES has been successfully proven by other polyols. The enzymatic hydrolysate of ternary DES-pretreated CS fermented over our genetically engineered yeast strain SFA1OE gave a high ethanol yield of 0.488 g/g total reducing sugar, demonstrating the effectiveness of the polyol-assisted ternary DES pretreatment in achieving high-efficiency cellulosic ethanol production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Xilanos / Zea mays / Etanol / Fermentación / Disolventes Eutécticos Profundos / Lignina Idioma: En Revista: Carbohydr Polym Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Xilanos / Zea mays / Etanol / Fermentación / Disolventes Eutécticos Profundos / Lignina Idioma: En Revista: Carbohydr Polym Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido