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Prediction strategy for screening functional Haloarchaea strains with qPCR assays.
Hu, Xinyu; Sun, Wenxiang; Zhang, Meng; Guo, Wenjun; Yang, Shujing; Zhu, Lin; Xiao, Xiang; Xu, Xiangru; Wei, Wei.
Afiliación
  • Hu X; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
  • Sun W; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
  • Zhang M; School of Agricultural Engineering, Jiangsu University, Zhenjiang, China.
  • Guo W; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
  • Yang S; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
  • Zhu L; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
  • Xiao X; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
  • Xu X; School of Agricultural Engineering, Jiangsu University, Zhenjiang, China.
  • Wei W; School of Agricultural Engineering, Jiangsu University, Zhenjiang, China. Electronic address: weiwei7096@ujs.edu.cn.
J Microbiol Methods ; 226: 107029, 2024 Nov.
Article en En | MEDLINE | ID: mdl-39245367
ABSTRACT
As an extremophile resource, functional Haloarchaea strains are extremely time-consuming to screen. Here, taking the screening of low-salt-tolerant strains as an example, based on the qPCR assays that shortened time by 4-7 times and achieved 100 % accuracy, a universal strategy for rapid and accurate screening of functional Haloarchaea strains was established.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena en Tiempo Real de la Polimerasa Idioma: En Revista: J Microbiol Methods Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena en Tiempo Real de la Polimerasa Idioma: En Revista: J Microbiol Methods Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos