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Nanoencapsulation enhances the antimicrobial and antioxidant stability of cyclic lipopeptides for controlling Fusarium graminearum.
Yi, Yanjie; Shan, Youtian; Luan, Pengyu; Sun, Zhongke; Wu, Xingquan; Ning, Zhiwen; Chen, Zhengkun; Zhang, Yunxiang; Zhao, Shuyun; Li, Chengwei.
Afiliación
  • Yi Y; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; Food Laboratory of Zhongyuan, Luohe, 462300, China; The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, 450001, China. Electronic address: yiyanjie@haut.edu.cn.
  • Shan Y; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; Vazyme Biotech Co., Ltd, Nanjing, 210000, China.
  • Luan P; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; Food Laboratory of Zhongyuan, Luohe, 462300, China; The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, 450001, China.
  • Sun Z; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, 450001, China.
  • Wu X; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, 450001, China.
  • Ning Z; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, 450001, China.
  • Chen Z; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, 450001, China.
  • Zhang Y; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, 450001, China.
  • Zhao S; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; The Key Laboratory of Functional Molecules for Biomedical Research, Zhengzhou, 450001, China.
  • Li C; School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; College of Life Science, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: lcw@haut.edu.cn.
Food Microbiol ; 124: 104621, 2024 Dec.
Article en En | MEDLINE | ID: mdl-39244372
ABSTRACT
Fusarium graminearum not only causes Fusarium head blight (FHB) on wheat but also produces fungal toxins that pose a serious threat to food safety. Biological control is one of the safe and most effective alternative methods. In this study, cyclic lipopeptides (CLPs) produced from Bacillus mojavensis B1302 were extracted and identified by LC-MS/MS. After preparing mesoporous silica nanoparticles-NH2 (MSNsN) and encapsulating CLPs, the characterization analysis showed that the interaction between CLPs and MSNsN enhanced the crystal structure of CLPs-MSNsN. The antimicrobial activity and antioxidant capacity of CLPs-MSNsN stored at 20 °C and 45 °C were decreased more slowly than those of free CLPs with increasing storage time, indicating the enhancement of the antimicrobial and antioxidant stability of CLPs. Moreover, the field control efficacy of long-term stored CLPs-MSNsN only decreased from 78.66% to 63.2%, but the efficacy of free CLPs decreased significantly from 84.34% to 26.01%. The deoxynivalenol (DON) content of wheat grains in the CLPs-MSNsN treatment group was lower than that in the free CLPs treatment group, which showed that long-term stored CLPs-MSNsN reduced the DON content in wheat grains. Further analysis of the action mechanism of CLPs-MSNsN on F. graminearum showed that CLPs-MSNsN could disrupt mycelial morphology, cause cell apoptosis, lead to the leakage of proteins and nucleic acids, and destroy the cell permeability of mycelia. This work puts a novel insight into the antimicrobial and antioxidant stability enhancement of CLPs-MSNsN through encapsulation and provides a potential fungicide to control F. graminearum, reduce toxins and ensure food safety.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Enfermedades de las Plantas / Triticum / Lipopéptidos / Fusarium / Antioxidantes Idioma: En Revista: Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Enfermedades de las Plantas / Triticum / Lipopéptidos / Fusarium / Antioxidantes Idioma: En Revista: Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido