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Engineered Halomonas for production of gamma-Aminobutyric acid and 2-Pyrrolidone.
Zhang, Ge; Guo, Weike; Yi, Xueqing; Zhang, Zhongnan; Zhang, Lizhan; Liu, Xu; Wu, Fuqing; Wu, Qiong; Chen, Guo-Qiang.
Afiliación
  • Zhang G; Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Guo W; Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
  • Yi X; Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
  • Zhang Z; Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
  • Zhang L; Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Liu X; Beijing PhaBuilder Biotechnology Co., LTD, Shunyi District, Beijing 101399, China.
  • Wu F; Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Beijing PhaBuilder Biotechnology Co., LTD, Shunyi District, Beijing 101399, China.
  • Wu Q; Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China; MOE Key Lab of Industrial Biocatalysts, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
  • Chen GQ; Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China; MOE Key Lab of Industrial Biocatalysts, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. Elec
Bioresour Technol ; : 131448, 2024 Sep 05.
Article en En | MEDLINE | ID: mdl-39244106
ABSTRACT
Gamma-Aminobutyric acid (GABA) is a derivative of L-glutamate, also a precursor for the synthesis of 2-pyrrolidone, which is a monomer of nylon-4. This study achieved a one-step biosynthesis of GABA and 2-pyrrolidone by Halomonas bluephagenesis overexpressing key genes involved in GABA and 2-pyrrolidone synthesis and deleting GABA degradation genes combined with reducing the degradation of 2-pyrrolidone precursor. The resulting H. bluephagenesis strain WLp07 was employed in whole-cell catalysis, producing 357 g/L of GABA and 72 wt% of PHA. Furthermore, a self-flocculating H. bluephagenesis allowed rapid, convenient recycling of the cells, achieving 880 g/L of GABA over three cycles. Shake flask studies showed that engineered H. bluephagenesis harboring ß-alanine CoA transferase was able to synthesized 2-pyrrolidone from GABA. H. bluephagenesis as a chassis of next generation industrial biotechnology (NGIB), demonstrated its diverse ability to produce GABA and 2-pyrrolidone in addition to intracellular PHA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido