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The role of bacterial size, shape and surface in macrophage engulfment of uropathogenic E. coli cells.
Peterson, Elizabeth; Söderström, Bill; Prins, Nienke; Le, Giang H B; Hartley-Tassell, Lauren E; Evenhuis, Chris; Grønnemose, Rasmus Birkholm; Andersen, Thomas Emil; Møller-Jensen, Jakob; Iosifidis, Gregory; Duggin, Iain G; Saunders, Bernadette; Harry, Elizabeth J; Bottomley, Amy L.
Afiliación
  • Peterson E; Australian Institute for Microbiology & Infection, University of Technology Sydney, Australia.
  • Söderström B; Australian Institute for Microbiology & Infection, University of Technology Sydney, Australia.
  • Prins N; Australian Institute for Microbiology & Infection, University of Technology Sydney, Australia.
  • Le GHB; School of Life Sciences, University of Technology Sydney, Sydney, Australia.
  • Hartley-Tassell LE; Institute for Glycomics, Griffith University, Gold Coast, Australia.
  • Evenhuis C; Australian Institute for Microbiology & Infection, University of Technology Sydney, Australia.
  • Grønnemose RB; Research Unit of Clinical Microbiology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.
  • Andersen TE; Research Unit of Clinical Microbiology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.
  • Møller-Jensen J; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
  • Iosifidis G; Australian Institute for Microbiology & Infection, University of Technology Sydney, Australia.
  • Duggin IG; Australian Institute for Microbiology & Infection, University of Technology Sydney, Australia.
  • Saunders B; School of Life Sciences, University of Technology Sydney, Sydney, Australia.
  • Harry EJ; Australian Institute for Microbiology & Infection, University of Technology Sydney, Australia.
  • Bottomley AL; Australian Institute for Microbiology & Infection, University of Technology Sydney, Australia.
PLoS Pathog ; 20(9): e1012458, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39241059
ABSTRACT
Uropathogenic Escherichia coli (UPEC) can undergo extensive filamentation in the host during acute urinary tract infections (UTIs). It has been hypothesised that this morphological plasticity allows bacteria to avoid host immune responses such as macrophage engulfment. However, it is still unclear what properties of filaments are important in macrophage-bacteria interactions. The aim of this work was to investigate the contribution of bacterial biophysical parameters, such as cell size and shape, and physiological parameters, such as cell surface and the environment, to macrophage engulfment efficiency. Viable, reversible filaments of known lengths and volumes were produced in the UPEC strain UTI89 using a variety of methods, including exposure to cell-wall targeting antibiotics, genetic manipulation and isolation from an in vitro human bladder cell model. Quantification of the engulfment ability of macrophages using gentamicin-protection assays and fluorescence microscopy demonstrated that the ability of filaments to avoid macrophage engulfment is dependent on a combination of size (length and volume), shape, cell surface and external environmental factors. UTI89 filamentation and macrophage engulfment efficiency were also found to occur independently of the SOS-inducible filamentation genes, sulA and ymfM in both in vivo and in vitro models of infection. Compared to filaments formed via antibiotic inhibition of division, the infection-derived filaments were preferentially targeted by macrophages. With several strains of UPEC now resistant to current antibiotics, our work identifies the importance of bacterial physiological and morphological states during infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: PLoS Pathog Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: PLoS Pathog Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos