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Proteomic analysis and in vivo visualization of extracellular vesicles from mouse oviducts during pre-implantation embryo development.
Stephens, Kalli K; Finnerty, Ryan M; Grant, DeAna G; Winuthayanon, Sarayut; Martin-DeLeon, Patricia A; Winuthayanon, Wipawee.
Afiliación
  • Stephens KK; Division of Animal Sciences, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, Missouri, USA.
  • Finnerty RM; Department of OB/GYN & Women's Health, School of Medicine, University of Missouri, Columbia, Missouri, USA.
  • Grant DG; Translational Biosciences Program, School of Medicine, University of Missouri, Columbia, Missouri, USA.
  • Winuthayanon S; Electron Microscopy Core Facility, University of Missouri, Columbia, Missouri, USA.
  • Martin-DeLeon PA; Division of Animal Sciences, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, Missouri, USA.
  • Winuthayanon W; Department of Biological Sciences, University of Delaware, Newark, Delaware, USA.
FASEB J ; 38(17): e70035, 2024 Sep 15.
Article en En | MEDLINE | ID: mdl-39239798
ABSTRACT
Pre-implantation embryonic development occurs in the oviduct during the first few days of pregnancy. The presence of oviductal extracellular vesicles (oEVs, also called oviductosomes) is crucial for pre-implantation embryonic development in vivo as oEVs often contain molecular transmitters such as proteins. Therefore, evaluating oEV cargo during early pregnancy could provide insights into factors required for proper early embryonic development that are missing in the current in vitro embryo culture setting. In this study, we isolated oEVs from the oviductal fluid at estrus and different stages of early embryonic development. The 2306-3066 proteins in oEVs identified at the different time points revealed 58-60 common EV markers identified in exosome databases. Oviductal extracellular vesicle proteins from pregnant samples significantly differed from those in non-pregnant samples. In addition, superovulation changes the protein contents in oEVs compared to natural ovulation at estrus. Importantly, we have identified that embryo-protectant proteins such as high-mobility protein group B1 and serine (or cysteine) peptidase inhibitor were only enriched in the presence of embryos. We also visualized the physical interaction of EVs and the zona pellucida of 4- to 8-cell stage embryos using transmission electron microscopy as well as in vivo live imaging of epithelial cell-derived GFP-tagged CD9 mouse model. All protein data in this study are readily available to the scientific community in a searchable format at https//genes.winuthayanon.com/winuthayanon/oviduct_ev_proteins/. In conclusion, we identified oEVs proteins that could be tested to determine whether they can improve embryonic developmental outcomes in vivo and in vitro setting.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteómica / Desarrollo Embrionario / Vesículas Extracelulares Límite: Animals / Pregnancy Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteómica / Desarrollo Embrionario / Vesículas Extracelulares Límite: Animals / Pregnancy Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos