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Cell-Int: a cell-cell interaction assay to identify native membrane protein interactions.
Aymoz-Bressot, Thibaud; Canis, Marie; Meurisse, Florian; Wijkhuisen, Anne; Favier, Benoit; Mousseau, Guillaume; Dupressoir, Anne; Heidmann, Thierry; Bacquin, Agathe.
Afiliación
  • Aymoz-Bressot T; CNRS UMR9196, Laboratory of Molecular Physiology and Pathology of Endogenous and Infectious Retroviruses, Gustave Roussy, Université Paris-Saclay, Villejuif, France.
  • Canis M; CNRS UMR9196, Laboratory of Molecular Physiology and Pathology of Endogenous and Infectious Retroviruses, Gustave Roussy, Université Paris-Saclay, Villejuif, France.
  • Meurisse F; VIROXIS, Gustave Roussy, Villejuif, France.
  • Wijkhuisen A; Université Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases (IMVA-HB/IDMIT), Paris, France.
  • Favier B; Université Paris-Saclay, CEA, INRAE, Médicaments et Technologies pour la Santé (MTS), Gif-sur-Yvette, France.
  • Mousseau G; Université Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases (IMVA-HB/IDMIT), Paris, France.
  • Dupressoir A; VIROXIS, Gustave Roussy, Villejuif, France.
  • Heidmann T; CNRS UMR9196, Laboratory of Molecular Physiology and Pathology of Endogenous and Infectious Retroviruses, Gustave Roussy, Université Paris-Saclay, Villejuif, France.
  • Bacquin A; CNRS UMR9196, Laboratory of Molecular Physiology and Pathology of Endogenous and Infectious Retroviruses, Gustave Roussy, Université Paris-Saclay, Villejuif, France.
Life Sci Alliance ; 7(11)2024 Nov.
Article en En | MEDLINE | ID: mdl-39237366
ABSTRACT
Intercellular protein-protein interactions (PPIs) have pivotal roles in biological functions and diseases. Membrane proteins are therefore a major class of drug targets. However, studying such intercellular PPIs is challenging because of the properties of membrane proteins. Current methods commonly use purified or modified proteins that are not physiologically relevant and hence might mischaracterize interactions occurring in vivo. Here, we describe Cell-Int a cell interaction assay for studying plasma membrane PPIs. The interaction signal is measured through conjugate formation between two populations of cells each expressing either a ligand or a receptor. In these settings, membrane proteins are in their native environment thus being physiologically relevant. Cell-Int has been applied to the study of diverse protein partners, and enables to investigate the inhibitory potential of blocking antibodies, as well as the retargeting of fusion proteins for therapeutic development. The assay was also validated for screening applications and could serve as a platform for identifying new protein interactors.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Unión Proteica / Comunicación Celular / Membrana Celular / Mapeo de Interacción de Proteínas / Proteínas de la Membrana Límite: Animals / Humans Idioma: En Revista: Life Sci Alliance Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Unión Proteica / Comunicación Celular / Membrana Celular / Mapeo de Interacción de Proteínas / Proteínas de la Membrana Límite: Animals / Humans Idioma: En Revista: Life Sci Alliance Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos