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Effect of Kruppel-like factor 4 on PTZ-induced acute seizure mice.
Li, Bingjin; Piao, Jingjing; Piao, Xinmiao; Geng, Zihui; Cheng, Ziqian; Zou, Xiaohan; Jiang, Huiyi.
Afiliación
  • Li B; Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, Second Hospital of Jilin University, Changchun, People's Republic of China.
  • Piao J; Department of Medical Research Centar, Second Hospital of Jilin University, Changchun, People's Republic of China.
  • Piao X; Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, Second Hospital of Jilin University, Changchun, People's Republic of China.
  • Geng Z; Department of Medical Research Centar, Second Hospital of Jilin University, Changchun, People's Republic of China.
  • Cheng Z; Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, Second Hospital of Jilin University, Changchun, People's Republic of China.
  • Zou X; Department of Medical Research Centar, Second Hospital of Jilin University, Changchun, People's Republic of China.
  • Jiang H; Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, Second Hospital of Jilin University, Changchun, People's Republic of China.
J Cell Mol Med ; 28(17): e18578, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39234952
ABSTRACT
Kruppel-like factor 4 (Klf4) is a transcription factor that is involved in neuronal regeneration and the development of glutamatergic systems. However, it is unknown whether Klf4 is involved in acute seizure. To investigate the potential role of Klf4 in pentylenetetrazol (PTZ)-induced seizure, western blotting, immunofluorescence, behaviour test and electrophysiology were conducted in this study. We found that Klf4 protein and mRNA expression were increased in both the hippocampus (HP) and prefrontal cortex (PFC) after PTZ-induced seizure in mice. HP-specific knockout (KO) of Klf4 in mice decreased protein expression of Klf4 and the down-stream Klf4 target tumour protein 53 (TP53/P53). These molecular changes are accompanied by increased seizure latency, reduced immobility time in the forced swimming test and tail suspension test. Reduced hippocampal protein levels for synaptic proteins, including glutamate receptor 1 (GRIA1/GLUA1) and postsynaptic density protein 95 (DLG4/PSD95), were also observed after Klf4-KO, while increased mRNA levels of complement proteins were observed for complement component 1q subcomponent A (C1qa), complement component 1q subcomponent B (C1qb), complement component 1q subcomponent C (C1qc), complement component 3 (C3), complement component 4A (C4a) and complement component 4B (C4b). Moreover, c-Fos expression induced by PTZ was reduced by hippocampal conditional KO of Klf4. Electrophysiology showed that PTZ-induced action potential frequency was decreased by overexpression of Klf4. In conclusion, these findings suggest that Klf4 plays an important role in regulating PTZ-induced seizures and therefore constitutes a new molecular target that should be explored for the development of antiepileptic drugs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pentilenotetrazol / Convulsiones / Ratones Noqueados / Factores de Transcripción de Tipo Kruppel / Factor 4 Similar a Kruppel / Hipocampo Límite: Animals Idioma: En Revista: J Cell Mol Med Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pentilenotetrazol / Convulsiones / Ratones Noqueados / Factores de Transcripción de Tipo Kruppel / Factor 4 Similar a Kruppel / Hipocampo Límite: Animals Idioma: En Revista: J Cell Mol Med Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido