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Preclinical Evidence for the Protective Capacity of Antibodies Induced by Lyme Vaccine Candidate VLA15 in People.
Lundberg, Urban; Hochreiter, Romana; Timofoyeva, Yekaterina; Kanevsky, Isis; Meinke, Andreas; Anderson, Annaliesa S; Simon, Raphael.
Afiliación
  • Lundberg U; Valneva Austria GmbH, Vienna, Austria.
  • Hochreiter R; Valneva Austria GmbH, Vienna, Austria.
  • Timofoyeva Y; Vaccine Research and Development, Pfizer Inc., Pearl River, New York, USA.
  • Kanevsky I; Vaccine Research and Development, Pfizer Inc., Pearl River, New York, USA.
  • Meinke A; Valneva Austria GmbH, Vienna, Austria.
  • Anderson AS; Vaccine Research and Development, Pfizer Inc., Pearl River, New York, USA.
  • Simon R; Vaccine Research and Development, Pfizer Inc., Pearl River, New York, USA.
Open Forum Infect Dis ; 11(9): ofae467, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39233712
ABSTRACT

Background:

Vaccine candidate VLA15 is designed to protect against the dominant Borrelia genospecies-causing Lyme disease in North America and Europe. Active immunization with VLA15 has protected in the mouse model of tick challenge. VLA15 is currently under evaluation in clinical studies for the prevention of Lyme borreliosis.

Methods:

Mice were passively administered sera from clinical trial participants vaccinated with VLA15, or normal human serum from unvaccinated individuals as control. Posttransfer serum anti-outer surface protein A (OspA) immunoglobulin G titers were assessed by enzyme-linked immunosorbent assay. Following passive transfer, mice were challenged with Ixodes ticks colonized with Borrelia burgdorferi (OspA serotype 1) or Borrelia afzelii (OspA serotype 2) and infection was determined by serology for VlsE C6 or by polymerase chain reaction and culture to assess the presence of Borrelia bacteria.

Results:

Passive transfer of immune sera prevented transmission of Borrelia from the tick vector and protected mice against challenge. Posttransfer protective threshold immunoglobulin G antibody titers were observed in this animal model of 131 U/mL for B burgdorferi (OspA serotype 1) and 352 U/mL for B afzelii (serotype 2).

Conclusions:

Passive transfer of sera from trial participants immunized with VLA15 protected mice from borreliosis in a tick challenge model. This indicates that VLA15 induces functional immune responses in people that can be linked to efficacy in a stringent preclinical model.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Open Forum Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Open Forum Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Estados Unidos