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Enhancing human milk studies: Introducing a less invasive human milk collection technique for the measurement of fatty acids.
Morniroli, Daniela; Vizzari, Giulia; Turolo, Stefano; Syren, Eva M L; Mazzocchi, Alessandra; Milani, Gregorio P; Fumagalli, Monica; Giannì, Maria L; Agostoni, Carlo.
Afiliación
  • Morniroli D; Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.
  • Vizzari G; Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, NICU, Milan, Italy.
  • Turolo S; Dialysis and Transplant Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Pediatric Nephrology, Milan, Italy.
  • Syren EML; Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.
  • Mazzocchi A; Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.
  • Milani GP; Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.
  • Fumagalli M; Pediatric Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
  • Giannì ML; Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.
  • Agostoni C; Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, NICU, Milan, Italy.
J Pediatr Gastroenterol Nutr ; 2024 Sep 04.
Article en En | MEDLINE | ID: mdl-39233525
ABSTRACT

OBJECTIVES:

The fatty acid supply of human milk (HM) contributes to health outcomes. Sampling fresh human milk to analyze its fatty acid content is challenging because of its ever-changing nature. Also, obtaining samples from lactating mothers is challenging. Facilitating HM collection and analysis is therefore an advantage.

METHODS:

We have conducted a study to validate a new method for obtaining HM samples for fatty acid analysis, using biological fluid sample collection pretreated sheets to adsorb drops of milk (Whatman 903 BHT-pretreated biological fluid collection sheet) as an alternative approach to collecting expressed milk. The study population included lactating mothers, enrolled between 24 and 96 h after delivery.

RESULTS:

A total of 124 breastmilk samples were analyzed using the two distinct approaches. The results of the free milk analysis were comparable to the analysis of adsorbed milk samples. The fatty acid families saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), omega-3, and omega-6 had r2 values of 0.93, 0.91, 0.91, 0.86, and 0.90, respectively. Bland-Altman plots showed a high agreement between fresh and adsorbed milk samples for SFA, MUFA, PUFA, omega-3, and omega-6 with a mean bias <2% and 95% limits of agreement within -5% and +5%.

CONCLUSIONS:

The results show no significant differences in fatty acid composition between fresh and adsorbed milk samples, suggesting the new method is equally effective in collecting representative samples for analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Pediatr Gastroenterol Nutr Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Pediatr Gastroenterol Nutr Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos