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Genetic evolutionary analysis of a strain of Senecavirus A in Anhui and the establishment of its detection method.
Li, Liang; Wang, Xuan; Chen, Lijun; Li, Jie; Xue, Yuting; Lin, Haicheng; Sun, HuiHui; Bo, Zongyi; Shen, Haixiao; Sun, Pei.
Afiliación
  • Li L; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
  • Wang X; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
  • Chen L; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
  • Li J; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
  • Xue Y; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
  • Lin H; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
  • Sun H; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
  • Bo Z; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
  • Shen H; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
  • Sun P; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Joint Research Center for Food Nutrition and Health of IHM, Anhui Agricultural University, Hefei, PR China. Electronic address: sunpei@ahau.edu.cn.
Infect Genet Evol ; 124: 105665, 2024 Sep 02.
Article en En | MEDLINE | ID: mdl-39233257
ABSTRACT

BACKGROUND:

Senecavirus A (SVA) is the only member of the genus Senecavirus in the family Picornaviridae, and is one of the pathogens of porcine blistering disease. SVA has been reported in the United States, Canada, China, Thailand, and Colombia.

METHODS:

In this study, positive SVA infection was detected by RT-PCR in sick materials collected from pig farms of different sizes in Anhui Province.

RESULTS:

In this study, a virulent strain of SVA was successfully obtained by viral isolation on BHK21 cells and named SVA-CH-AHAU-1. Meanwhile, a simple, rapid and accurate nano-PCR method for the detection of SVA infection was established in this study, using the recombinant plasmid pClone-SVA-3D as a template.

CONCLUSIONS:

The complete genome of SVA-CH-AHAU-1 is 7286 bp, including a 5' non-coding region (UTR), an open reading frame (ORF) of 6546 nucleotides, encoding 2182 amino acids (aa), and a 3' UTR with Poly(A) features, and phylogenetic analysis showed that this isolate had the highest nucleotide homology (97.9 %) with the US isolate US-15-41901SD. In this study, the virulent strain SVA-CH-AHAU-1 was found to recombine in the ORF region with isolates SVA-CH-SDGT-2017 and SVA/Canada/ON/FMA-2015-0024 T2/2015. The complete genome has been submitted to GeneBank with the accession number OM654411. In addition, our results suggest that the established nano-PCR assay can be used as an economical, reliable and sensitive method for the field diagnosis of SVA method, especially in resource-limited areas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Infect Genet Evol Asunto de la revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Infect Genet Evol Asunto de la revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos