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Development and characterization of a novel poly(N-isopropylacrylamide)-based thermoresponsive photoink and its applications in DLP bioprinting.
Perera, Kalindu D C; Boiani, Sophia M; Vasta, Alexandra K; Messenger, Katherine J; Delva, Sabrina; Menon, Jyothi U.
Afiliación
  • Perera KDC; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA. jmenon@uri.edu.
  • Boiani SM; Department of Chemical Engineering, College of Engineering, University of Rhode Island, Kingston, RI 02881, USA.
  • Vasta AK; Department of Chemical Engineering, College of Engineering, University of Rhode Island, Kingston, RI 02881, USA.
  • Messenger KJ; Department of Biomedical Engineering, College of Engineering, University of Rhode Island, Kingston, RI 02881, USA.
  • Delva S; Department of Biomedical Engineering, College of Engineering, University of Rhode Island, Kingston, RI 02881, USA.
  • Menon JU; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA. jmenon@uri.edu.
J Mater Chem B ; 12(38): 9767-9779, 2024 Oct 02.
Article en En | MEDLINE | ID: mdl-39230440
ABSTRACT
The field of 3-dimensional (3D) bioprinting has significantly expanded capabilities in producing precision-engineered hydrogel constructs, and recent years have seen the development of various stimuli-responsive bio- and photoinks. There is, however, a distinct lack of digital light processing (DLP)-compatible photoinks with thermoresponsivity. To remedy this, this work focuses on formulating and optimizing a versatile ink for DLP printing of thermoresponsive hydrogels, with numerous potential applications in tissue engineering, drug delivery, and adjacent biomedical fields. Photoink optimization was carried out using a multifactorial study design. The optimized photoink yielded crosslinked hydrogels with strong variations in hydrophobicity (contact angles of 44.4° LCST), indicating marked thermoresponsivity. Mechanical- and rheological characterization of the printed hydrogels showed significant changes above the LCST storage- and loss moduli both increased and loss tangent and compressive modulus decreased above this temperature (P ≤ 0.01). The highly cytocompatible hydrogel microwell arrays yielded both single- and multilayer spheroids with human dermal fibroblasts (HDFs) and HeLa cells successfully. Evaluation of the release of encapsulated model macro- (bovine serum albumin, BSA) and small molecule (rhodamine B) drugs in a buffer solution showed an interestingly inverted thermoresponsive release profile with >80% release at room temperature and about 50-60% release above the gels' LCST. All told, the optimized ink holds great promise for multiple biomedical applications including precise and high-resolution fabrication of complex tissue structures, development of smart drug delivery systems and 3D cell culture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resinas Acrílicas / Hidrogeles / Bioimpresión Límite: Animals / Humans Idioma: En Revista: J Mater Chem B Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resinas Acrílicas / Hidrogeles / Bioimpresión Límite: Animals / Humans Idioma: En Revista: J Mater Chem B Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido