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Purifying selection drives distinctive arsenic metabolism pathways in prokaryotic and eukaryotic microbes.
Li, Lijuan; Chen, Songcan; Xue, Ximei; Chen, Jieyin; Tian, Jian; Huo, Lijuan; Zhang, Tuo; Zeng, Xibai; Su, Shiming.
Afiliación
  • Li L; Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agricultural Environment, MARA, Beijing 100081, P.R. China.
  • Chen S; Division of Microbial Ecology, Center for Microbiology and Environmental Systems Science, University of Vienna, Vienna 1030, Austria.
  • Xue X; Institute of Urban Environment, Key Laboratory of Urban Environment and Health, Chinese Academy of Sciences, Xiamen 361021, P.R. China.
  • Chen J; Institute of Plant Protection, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
  • Tian J; Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
  • Huo L; School of Environment and Resources, Taiyuan University of Science and Technology, Taiyuan 030024, P.R. China.
  • Zhang T; School of Environmental and Life Science, Nanning Normal University, Nanning 530100, P.R. China.
  • Zeng X; Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agricultural Environment, MARA, Beijing 100081, P.R. China.
  • Su S; Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agricultural Environment, MARA, Beijing 100081, P.R. China.
ISME Commun ; 4(1): ycae106, 2024 Jan.
Article en En | MEDLINE | ID: mdl-39229495
ABSTRACT
Microbes play a crucial role in the arsenic biogeochemical cycle through specific metabolic pathways to adapt to arsenic toxicity. However, the different arsenic-detoxification strategies between prokaryotic and eukaryotic microbes are poorly understood. This hampers our comprehension of how microbe-arsenic interactions drive the arsenic cycle and the development of microbial methods for remediation. In this study, we utilized conserved protein domains from 16 arsenic biotransformation genes (ABGs) to search for homologous proteins in 670 microbial genomes. Prokaryotes exhibited a wider species distribution of arsenic reduction- and arsenic efflux-related genes than fungi, whereas arsenic oxidation-related genes were more prevalent in fungi than in prokaryotes. This was supported by significantly higher acr3 (arsenite efflux permease) expression in bacteria (upregulated 3.72-fold) than in fungi (upregulated 1.54-fold) and higher aoxA (arsenite oxidase) expression in fungi (upregulated 5.11-fold) than in bacteria (upregulated 2.05-fold) under arsenite stress. The average values of nonsynonymous substitutions per nonsynonymous site to synonymous substitutions per synonymous site (dN/dS) of homologous ABGs were higher in archaea (0.098) and bacteria (0.124) than in fungi (0.051). Significant negative correlations between the dN/dS of ABGs and species distribution breadth and gene expression levels in archaea, bacteria, and fungi indicated that microbes establish the distinct strength of purifying selection for homologous ABGs. These differences contribute to the distinct arsenic metabolism pathways in prokaryotic and eukaryotic microbes. These observations facilitate a significant shift from studying individual or several ABGs to characterizing the comprehensive microbial strategies of arsenic detoxification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ISME Commun Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ISME Commun Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido