The yin and yang of the universal transcription factor NusG.

Delbeau, Madeleine; Froom, Ruby; Landick, Robert; Darst, Seth A; Campbell, Elizabeth A

Delbeau, Madeleine; Froom, Ruby; Landick, Robert; Darst, Seth A; Campbell, Elizabeth A.

Afiliación

Delbeau M; Laboratory of Molecular Pathogenesis, The Rockefeller University, New York, NY 10065, USA.
Froom R; Laboratory of Molecular Pathogenesis, The Rockefeller University, New York, NY 10065, USA.
Landick R; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Darst SA; Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY 10065, USA.
Campbell EA; Laboratory of Molecular Pathogenesis, The Rockefeller University, New York, NY 10065, USA. Electronic address: campbee@rockefeller.edu.

Curr Opin Microbiol ; 81: 102540, 2024 Oct.

Article en En | MEDLINE | ID: mdl-39226817

ABSTRACT

ABSTRACT

RNA polymerase (RNAP), the central enzyme of transcription, intermittently pauses during the elongation stage of RNA synthesis. Pausing provides an opportunity for regulatory events such as nascent RNA folding or the recruitment of transregulators. NusG (Spt5 in eukaryotes and archaea) regulates RNAP pausing and is the only transcription factor conserved across all cellular life. NusG is a multifunctional protein its N-terminal domain (NGN) binds to RNAP, and its C-terminal KOW domain in bacteria interacts with transcription regulators such as ribosomes and termination factors. In Escherichia coli, NusG acts as an antipausing factor. However, recent studies have revealed that NusG has distinct transcriptional regulatory roles specific to bacterial clades with clinical implications. Here, we focus on NusG's dual roles in the regulation of pausing.

Asunto(s)

Proteínas de Escherichia coli; Escherichia coli; Factores de Elongación de Péptidos; Proteínas de Escherichia coli/metabolismo; Proteínas de Escherichia coli/genética; Factores de Elongación de Péptidos/metabolismo; Factores de Elongación de Péptidos/genética; Escherichia coli/genética; Escherichia coli/metabolismo; Factores de Transcripción/metabolismo; Factores de Transcripción/genética; ARN Polimerasas Dirigidas por ADN/metabolismo; ARN Polimerasas Dirigidas por ADN/genética; Factores de Elongación Transcripcional/metabolismo; Factores de Elongación Transcripcional/genética; Regulación Bacteriana de la Expresión Génica; Transcripción Genética; Bacterias/genética; Bacterias/metabolismo

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Elongación de Péptidos / Proteínas de Escherichia coli / Escherichia coli Idioma: En Revista: Curr Opin Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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