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Paracoccidioides lutzii Infects Galleria mellonella Employing Formamidase as a Virulence Factor.
Pereira, Elisa Dias; Moreira, Thalison Rodrigues; Cruz-Leite, Vanessa Rafaela Milhomem; Tomazett, Mariana Vieira; Souza Silva, Lana O'Hara; Graziani, Daniel; Martins, Juliana Assis; Amaral, André Corrêa; Weber, Simone Schneider; Parente-Rocha, Juliana Alves; Soares, Célia Maria de Almeida; Borges, Clayton Luiz.
Afiliación
  • Pereira ED; Department of Biochemistry and Molecular Biology, Institute of Biological Sciences II, Federal University of Goiás, Goiânia, Brazil.
  • Moreira TR; Department of Biochemistry and Molecular Biology, Institute of Biological Sciences II, Federal University of Goiás, Goiânia, Brazil.
  • Cruz-Leite VRM; Department of Biochemistry and Molecular Biology, Institute of Biological Sciences II, Federal University of Goiás, Goiânia, Brazil.
  • Tomazett MV; Department of Biochemistry and Molecular Biology, Institute of Biological Sciences II, Federal University of Goiás, Goiânia, Brazil.
  • Souza Silva LO; Department of Biochemistry and Molecular Biology, Institute of Biological Sciences II, Federal University of Goiás, Goiânia, Brazil.
  • Graziani D; Multiuser Laboratory for the Evaluation of Molecules, Cells and Tissues, Federal University of Goiás, Goiânia, Brazil.
  • Martins JA; Laboratory of Nano&Biotechnology, Department of Biotechnology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Brazil.
  • Amaral AC; Laboratory of Nano&Biotechnology, Department of Biotechnology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Brazil.
  • Weber SS; Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
  • Parente-Rocha JA; Department of Biochemistry and Molecular Biology, Institute of Biological Sciences II, Federal University of Goiás, Goiânia, Brazil.
  • Soares CMA; Department of Biochemistry and Molecular Biology, Institute of Biological Sciences II, Federal University of Goiás, Goiânia, Brazil.
  • Borges CL; Department of Biochemistry and Molecular Biology, Institute of Biological Sciences II, Federal University of Goiás, Goiânia, Brazil.
PLoS Negl Trop Dis ; 18(9): e0012452, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39226308
ABSTRACT
The formamidase (FMD) enzyme plays an important role in fungal thriving by releasing a secondary nitrogen source as a product of its activity. In Paracoccidioides species, previous studies have demonstrated the upregulation of this enzyme in a wide range of starvation and infective-like conditions. However, Paracoccidioides lutzii formamidase has not yet been defined as a virulence factor. Here, by employing in vivo infections using an fmd-silenced strain in Galleria mellonella larvae model, we demonstrate the influence of formamidase in P. lutzii's immune stimulation and pathogenicity. The formamidase silencing resulted in improper arrangement of the nodules, poor melanogenesis and decreased fungal burden. Thus, we suggest that formamidase may be a piece composing the process of molecular recognition by Galleria immune cells. Furthermore, formamidase silencing doubled the observed survival rate of the larvae, demonstrating its importance in fungal virulence in vivo. Therefore, our findings indicate that formamidase contributes to Galleria's immune incitement and establishes the role of this enzyme as a P. lutzii virulence factor.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paracoccidioides / Factores de Virulencia / Larva / Mariposas Nocturnas Límite: Animals Idioma: En Revista: PLoS Negl Trop Dis Asunto de la revista: MEDICINA TROPICAL Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paracoccidioides / Factores de Virulencia / Larva / Mariposas Nocturnas Límite: Animals Idioma: En Revista: PLoS Negl Trop Dis Asunto de la revista: MEDICINA TROPICAL Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos